This is a position in Dr. Andrew Adams Lab within the Department of Surgery at the University of
Minnesota. We are looking for a motivated researcher with a strong interest in basic and translational
clinical research. A background in immunology or a related field (molecular biology, virology, genetics,
etc) is a bonus! This position works under the direction of our higher-level researchers in the lab and
under supervision of Dr. Andrew Adams and Dr. Neal Iwakoshi. The purpose of the Researcher 1/2
position is to assist in these areas of investigation of our lab: (1) costimulation blockade and costimulation
independent rejection (2) xenotransplantation (3) transplant diagnostics (4) transplant outcomes.
This is a great opportunity for a BA/BS level scientist to join a cutting-edge academic laboratory focused
on developing novel cellular and molecular therapies to prevent graft rejection and induce immune
transplant tolerance.
Job Duties for 8351RL & 8352R2 (Researcher 1 & 2):
Perform a variety of basic molecular and biochemical analyses on animal specimens, including
fluorescence microscopy analyses, flow cytometry, cell isolation, freeze/thaw of archived cells,
cell culture; flow cytometric characterization and purification (sorting); biochemical assays on
DNA, RNA/protein. Preparation of media and various molecular biology techniques.
Collect and integrate data from multiple sources. Review literature for relevant sources of
scientific information and methods.
Mouse colony management, such as: breeding, checking for new liters, weaning infant mice.
Maintain laboratory equipment, order and stock items.
Keep detailed records of all experiments and procedures in laboratory notebooks, databases,
spreadsheets, and web-based applications.
Conduct background literature research on relevant topics including current methods, reagents,
and ethics.
Prepare media, enzymes, and solutions under sterile conditions in large quantities.
Additional Job Duties for 8352R2 (Researcher 2):
Perform intermediate/advanced molecular and biochemical analyses on animal and human
specimens, including fluorescence microscopy analyses, flow cytometry, cell isolation,
freeze/thaw of archived cells, cell culture; flow cytometric characterization and purification
(sorting); biochemical assays on DNA, RNA/protein. Preparation of media and various molecular
biology techniques. These analyses are to be followed up with statistical analysis and preliminary
interpretation of results to identify trends or anomalies.
Help monitor and train undergraduate student workers and volunteers.
Oversee the use of laboratory equipment, training personnel/volunteers and maintaining
performance and maintenance records.
Draft sections of primary research manuscripts, prepare publication-quality figures, and provide
data summaries for grant applications.
Researcher 2s will generally be expected to perform tasks with less direction, more
independence and function as a resource for more junior researchers, volunteers and students.