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Title: Scientist II
Pay : 54/HR on W2
Location: 213 E. Grand Avenue, South San Francisco, CA 94080
Qualifications:
Education and experience required:
B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields
Key skills:
Maintain and propagate CHO cells in suspension culture independently
Perform transient and stable transfections for protein expression in mammalian cells(CHO cells).
Purification batch purification using bench top.
Candidate should have foundational experience in purification and QC
Must haves:
Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.
Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).
Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.
Nice to haves/preferred:
Experience with high-throughput systems and automation.
Experience with batch purification using a variety of scaffolds and basic QC.
Industry experience is preferred.
Software skills:
Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.
Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.
Skills/techniques needed:
Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.
Strong communication, organizational, and interpersonal skills.
Able to maintain a "can-do" attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs.
Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials
Able to troubleshoot cell culture system and think critically about experiments.
Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work.
Note:
Onsite role.
Lab based role.
No PHD.
Responsibilities:
Department summary:
We are Discovery Biologics, and we are on a quest for cures and are committed to be the world's premier, most research-intensive biopharmaceutical company. We identify and target steps in disease mechanisms or pathways that could be inhibited or enhanced. Our goal is to isolate a compound that is effective against a disease target. Using innovative thinking, state-of-the-art facilities and robust scientific methodology we collaborate to Client the next medical breakthrough.
Our teams combine leading drug discovery capabilities and world-class R&D with the purpose of turning breakthrough science into life-changing medicines.
We are looking for a motivated scientist to be an integral part of the Protein Science department within Discovery Biologics at Client Research Labs (MRL)- South San Francisco.
The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and Client protein modalities to enable all aspects of Early Discovery.
Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.
Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.
Job responsibilities in detail:
Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.
Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.
Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.
Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.
Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.
Maintain and troubleshoot mammalian cell culture systems.
Document protein production processes, prepare reports and communicate results
ELN documentation.
Kindly share your resume with answers:
Q1: How many years of exp in foundational experience in purification (using bench top) and QC
Q2: How many years of exp in Maintaining and propagate CHO cells in suspension culture independently
Q3: How many years of exp in Performing transient and stable transfections for protein expression in mammalian cells(CHO cells).
Q4: How far you live and how will you commute to South SFO daily