The individual will develop and optimize protein expression, purification, and characterization ... enzymes, ligand receptors, and recombinant immunogens from different research areas such as ...
The individual will develop and optimize protein expression, purification, and characterization ... enzymes, ligand receptors, and recombinant immunogens from different research areas such as ...
The individual will develop and optimize protein expression, purification, and characterization ... enzymes, ligand receptors, and recombinant immunogens from different research areas such as ...
The individual will develop and optimize protein expression, purification, and characterization ... enzymes, ligand receptors, and recombinant immunogens from different research areas such as ...
The individual will develop and optimize protein expression, purification, and characterization ... enzymes, ligand receptors, and recombinant immunogens from different research areas such as ...
The individual will develop and optimize protein expression, purification, and characterization ... enzymes, ligand receptors, and recombinant immunogens from different research areas such as ...
Research Associate
Chapel Hill, NC · On-site
Procedures include PCR, in vitro transcription, PAGE purification of RNAs, enzymatic reactions ... Previous work with RNA, and/or enzymology are preferred. Special Physical/Mental Requirements ...
Research Associate
Chapel Hill, NC · On-site
Procedures include PCR, in vitro transcription, PAGE purification of RNAs, enzymatic reactions ... Previous work with RNA, and/or enzymology are preferred. Special Physical/Mental Requirements ...
Own the strategy for E. coli host strain, vector, and expression-system for recombinant enzymes ... Partner with process development, analytical development, downstream purification, informatics ...
Own the strategy for E. coli host strain, vector, and expression-system for recombinant enzymes ... Partner with process development, analytical development, downstream purification, informatics ...
Own the strategy for E. coli host strain, vector, and expression-system for recombinant enzymes ... Partner with process development, analytical development, downstream purification, informatics ...
Own the strategy for E. coli host strain, vector, and expression-system for recombinant enzymes ... Partner with process development, analytical development, downstream purification, informatics ...
The Downstream Operations Scientist is responsible for leading downstream purification unit ... PAGE, enzyme activity and endotoxin assays to quantify yield, activity, and purity at various ...
The Downstream Operations Scientist is responsible for leading downstream purification unit ... PAGE, enzyme activity and endotoxin assays to quantify yield, activity, and purity at various ...
Research Scientist/Engr 1
Seattle, WA · On-site
$58.01K/yr
... purification, chromatography/FPLC workflows, and protein QC. • Biochemical assays: experience with enzyme assays, transcription assays, fluorescence-based readouts, or related in vitro functional ...
Research Scientist/Engr 1
Seattle, WA · On-site
$58.01K/yr
... purification, chromatography/FPLC workflows, and protein QC. • Biochemical assays: experience with enzyme assays, transcription assays, fluorescence-based readouts, or related in vitro functional ...
The Downstream Operations Scientist is responsible for leading downstream purification unit ... PAGE, enzyme activity and endotoxin assays to quantify yield, activity, and purity at various ...
The Downstream Operations Scientist is responsible for leading downstream purification unit ... PAGE, enzyme activity and endotoxin assays to quantify yield, activity, and purity at various ...
Research Scientist/Engr 1
$58.01K/yr
Protein production: bacterial expression, affinity purification, chromatography/FPLC workflows, and protein QC. Biochemical assays: experience with enzyme assays, transcription assays, fluorescence ...
Research Scientist/Engr 1
$58.01K/yr
Protein production: bacterial expression, affinity purification, chromatography/FPLC workflows, and protein QC. Biochemical assays: experience with enzyme assays, transcription assays, fluorescence ...
... Protein Purification, Analytics and Developability to support the build-out and day-to-day ... enzymes. * Operate and maintain automated chromatography systems, including Size Exclusion ...
... Protein Purification, Analytics and Developability to support the build-out and day-to-day ... enzymes. * Operate and maintain automated chromatography systems, including Size Exclusion ...
Design and execute enzymatic reactions with a focus on in vitro transcription and enzyme kinetics * Quality Control & Analysis: Perform comprehensive nucleic acid purification and characterization ...
Design and execute enzymatic reactions with a focus on in vitro transcription and enzyme kinetics * Quality Control & Analysis: Perform comprehensive nucleic acid purification and characterization ...
Design and execute enzymatic reactions with a focus on in vitro transcription and enzyme kinetics * Quality Control & Analysis: Perform comprehensive nucleic acid purification and characterization ...
Design and execute enzymatic reactions with a focus on in vitro transcription and enzyme kinetics * Quality Control & Analysis: Perform comprehensive nucleic acid purification and characterization ...
Senior Bioprocess Scientist / Engineer
Champaign, IL · On-site
$90K - $110K/yr
... purification processes across bacterial, yeast, and fungal hosts for a range of products including proteins, lipids, amino acids, organic acids, polysaccharides, hydrocolloids, and enzymes * Act as a ...
Senior Bioprocess Scientist / Engineer
Champaign, IL · On-site
$90K - $110K/yr
... purification processes across bacterial, yeast, and fungal hosts for a range of products including proteins, lipids, amino acids, organic acids, polysaccharides, hydrocolloids, and enzymes * Act as a ...
Lead Research Technologist - Feng Lab
Cleveland, OH · On-site
$79.73K/yr
The role includes assisting with small animal experiments involving viral infection and tumor formation, performing protein purification and enzyme assays, and conducting molecular manipulation of ...
Lead Research Technologist - Feng Lab
Cleveland, OH · On-site
$79.73K/yr
The role includes assisting with small animal experiments involving viral infection and tumor formation, performing protein purification and enzyme assays, and conducting molecular manipulation of ...
Postdoctoral Fellow or Staff Scientist - Protein Chemistry, Crystallography and Structure Determi...
Duarte, CA · On-site
$38.67 - $123.37/hr
... purification), crystallography (crystallization, optimization, data collection and structure determination), biochemistry (enzyme kinetics) and biophysics (Surface Plasmon Resonance, Iosthermal ...
Postdoctoral Fellow or Staff Scientist - Protein Chemistry, Crystallography and Structure Determi...
Duarte, CA · On-site
$38.67 - $123.37/hr
... purification), crystallography (crystallization, optimization, data collection and structure determination), biochemistry (enzyme kinetics) and biophysics (Surface Plasmon Resonance, Iosthermal ...
Postdoctoral Fellow or Staff Scientist - Protein Chemistry, Crystallography and Structure Determi...
$38.67 - $123.37/hr
... purification), crystallography (crystallization, optimization, data collection and structure determination), biochemistry (enzyme kinetics) and biophysics (Surface Plasmon Resonance, Iosthermal ...
Postdoctoral Fellow or Staff Scientist - Protein Chemistry, Crystallography and Structure Determi...
$38.67 - $123.37/hr
... purification), crystallography (crystallization, optimization, data collection and structure determination), biochemistry (enzyme kinetics) and biophysics (Surface Plasmon Resonance, Iosthermal ...
Biochemistry Tutor
Mount Pleasant, SC · Remote
$40/hr
... enzyme inhibition mechanisms, and bioenergetics calculations. Guides students through Lineweaver-Burk plot analysis, free energy calculations, protein purification strategies, and interpreting gel ...
Biochemistry Tutor
Mount Pleasant, SC · Remote
$40/hr
... enzyme inhibition mechanisms, and bioenergetics calculations. Guides students through Lineweaver-Burk plot analysis, free energy calculations, protein purification strategies, and interpreting gel ...
Biochemistry Tutor
Hempstead, NY · Remote
$40/hr
... enzyme inhibition mechanisms, and bioenergetics calculations. Guides students through Lineweaver-Burk plot analysis, free energy calculations, protein purification strategies, and interpreting gel ...
Biochemistry Tutor
Hempstead, NY · Remote
$40/hr
... enzyme inhibition mechanisms, and bioenergetics calculations. Guides students through Lineweaver-Burk plot analysis, free energy calculations, protein purification strategies, and interpreting gel ...
Biochemistry Tutor
NM · Remote
$40/hr
... enzyme inhibition mechanisms, and bioenergetics calculations. Guides students through Lineweaver-Burk plot analysis, free energy calculations, protein purification strategies, and interpreting gel ...
Biochemistry Tutor
NM · Remote
$40/hr
... enzyme inhibition mechanisms, and bioenergetics calculations. Guides students through Lineweaver-Burk plot analysis, free energy calculations, protein purification strategies, and interpreting gel ...
Enzyme Purification information
What are the key skills and qualifications needed to thrive as an Enzyme Purification Specialist, and why are they important?
What are some common challenges faced in enzyme purification roles, and how can they be addressed?
What is enzyme purification?
What is the difference between Enzyme Purification vs Protein Purification?
| Aspect | Enzyme Purification | Protein Purification |
|---|---|---|
| Credentials | Biochemist, Molecular Biologist | Biochemist, Molecular Biologist |
| Work Environment | Laboratories, biotech companies | Laboratories, biotech companies |
| Industry Usage | Enzyme isolation for research and industrial use | General protein isolation for various applications |
| Focus | Isolating and characterizing specific enzymes | Isolating and analyzing diverse proteins |
Enzyme Purification specifically involves isolating enzymes for research or industrial purposes, focusing on their activity and function. Protein Purification covers a broader range of proteins, including enzymes, structural proteins, and antibodies. While both roles require similar skills and work environments, enzyme purification emphasizes enzyme activity assays, whereas protein purification involves diverse techniques for different protein types.

Full-time
Medical, Dental, Vision, Life, Retirement, PTO
Posted 29 days ago
Revvity rating
9.5
Based on 8 frontline employees who took The Breakroom Quiz
Job description
Associate Scientist - Recombinant Protein Development
Location(s)
San Diego - BioLegend
About Us
Revvity is a developer and provider of end-to-end solutions designed to help scientists, researchers, and clinicians solve the world's greatest health challenges. We pair the enthusiasm of an industry disruptor with the experience of a longtime leader. Our team of 11,000+ colleagues from around the globe are vital to our success and the reason we're able to push boundaries in pursuit of better human health.
Find your future at Revvity
Job Summary
BioLegend is looking for an Associate Scientist to join the Recombinant Protein team. This position is responsible for developing functional protein products using cutting-edge protein expression and purification technologies. The individual will contribute to business revenue by transforming scientific knowledge into high-quality, sellable products.
The candidate should demonstrate a strong ability to self-start and adapt to the company's quality system. Duties include designing and implementing gene-to-protein workflows to produce high-quality recombinant proteins for use as research reagents in a fast-paced environment. The individual will develop and optimize protein expression, purification, and characterization processes across both prokaryotic and eukaryotic expression platforms. The candidate should possess excellent critical thinking and technical skills, along with sufficient troubleshooting experience to develop products efficiently while ensuring quality and safety. The individual will also participate in initiating sustainability efforts for existing designs based on market demand.
This position offers an excellent opportunity for technology exploration, scientific knowledge expansion, leadership development, and career growth within a leading biotechnology company.
The individual will perform laboratory-based protein processing, including cell lysis, refolding, and purification using various preparative chromatography methods, to develop final product formulations. They will actively collaborate with multidisciplinary teams to ensure the on-time delivery of internal and external custom orders and projects within the organization.
Essential Functions
Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
- Familiar with recombinant protein product expression platforms of various protein families such as cytokines, growth factors, enzymes, ligand receptors, and recombinant immunogens from different research areas such as carcinogenesis, immunology, neuroscience, etc.
- Implement bacterial protein expression culture and develop and run protein purification schemes using various chromatography methods on various platforms (i.e., AKTA FPLC, HPLC).
- Follow GDP (Good Document Practices) on processing records and conduct technical transfer under ISO9001:2008/ISO13485:2016 standards.
- Participate in product design and developmental stage review. Provide quality records to ensure traceability and risk control for product releasing.
- Own assigned product family in new product development and is responsible for driving projects to their completion.
- Support troubleshooting and optimization on protein expression, homogeneity, and stability of developing and released products.
- Work with the team and QA to execute and validate different protein processes, equipment and chemistry protocols.
- Support to facilitate Manufacturing Group for purification/QC workflow development and SOP optimizing/ troubleshooting in various production stages, including expression constructs, culture systems, purification schemes, formulation, and stability issues.
- Establish, coordinate, and communicate project timelines and deliverables with internal, and external team to provide weekly, monthly, and quarterly reports.
- Responsible for personnel training, goal setting and performance management.
- Maintain a current knowledge of protein chemistry, synthetic biology, and scientific literature.
- Other project responsibilities may be required.
Minimum Qualifications - Education and Experience
- B.S. degree with minimum 6 years' experience in Biotechnology industry
- M.S. degree with minimum 3 years' experience in Biotechnology industry
- 3+ years of hands-on experience with protein purification via various chromatography methods.
Preferred Qualifications - Education and Experience
- PhD degree in Life Sciences or related fields with Biotechnology industry experience with demonstrated quality system knowledge including ISO 13485.
- Experience in protein product production including protein expression and purification proteins
- Extensive experience in protein or antibody purification and modification.
- Extensive experience in process optimization for production scale up.
Work Environment & Physical Demands
Lab
While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.
The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job.
While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.
The base salary range for this full-time position is $90,000 - 100,000. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training. Your recruiter can share more about the specific salary range for your preferred location during the hiring process.
Please note that base pay is only one part of our total compensation package and is determined within a range. This range allows for the successful candidate to have an opportunity to progress within the position and develop at our company. This base pay range does not take into account bonuses, equity, or other benefits which may be applicable and are dependent on the level and position offered.
What do we offer?
We provide competitive and comprehensive benefits to our employees. Below are some highlights of our benefits:
- Medical, Dental, and Vision Insurance Options
- Life and Disability Insurance
- Paid Time-Off
- Parental Benefits
- Compassionate Care Leave
- 401k with Company Match
- Employee Stock Purchase Plan
Learn more about Revvity's benefits by visiting our Bswift page. Log-In instructions are provided towards the bottom of the Bswift page.
*For benefit-eligible roles only. Part-time and temporary roles may not be eligible for all benefits listed. Please reach out to your recruiter for more information.
Revvity is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to any characteristic or status protected by applicable federal, state, and/or local laws. If you are an applicant with a disability that requires reasonable accommodation to complete any part of the application process or are limited in the ability-or unable to use-the online application system and need an alternative method for applying, you may contact OpenJobs@revvity.com.
About Revvity
Sourced by ZipRecruiter
Industry
Biotechnology research and development
Company size
10,000+ Employees
Headquarters location
Waltham, MA, US
Year founded
2023