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2Nd Shift Phlebotomy Jobs (NOW HIRING)

Inpatient phlebotomy at Duke Regional Hospital in Durham. Monday - Friday, 12:30 p.m. - 9:00 p.m. and one in three weekends. This position is eligible for the second shift differential. General ...

Inpatient Phlebotomy - Second Shift

Durham, NC · On-site

$17.50 - $23/hr

Inpatient phlebotomy at Duke Regional Hospital in Durham. Monday - Friday, 12:30 p.m. - 9:00 p.m. and one in three weekends. This position is eligible for the second shift differential. General ...

Phlebotomist-Second Shift

Cincinnati, OH · On-site

$16.25 - $20.50/hr

Schedule: 3PM-11:30PM Shift Differential available! Responsibilities * Assist with study ... Demonstrate technical skills in phlebotomy, laboratory specimen collection, electrocardiogram ...

Phlebotomist-Second Shift

Cincinnati, OH · On-site +1

$16.25 - $20.50/hr

Schedule: 3PM-11:30PM Shift Differential available! Responsibilities * Assist with study ... Demonstrate technical skills in phlebotomy, laboratory specimen collection, electrocardiogram ...

Phlebotomist - 2nd shift

Mequon, WI · On-site

$15 - $18.75/hr

Phlebotomy certification or completed training program or 6 months or more experience as a phlebotomist Additional Job Standards: * In lieu of work experience, a Phlebotomy certification or completed ...

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2Nd Shift Phlebotomy information

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How much do 2nd shift phlebotomy jobs pay per hour?

As of Aug 5, 2026, the average hourly pay for 2nd shift phlebotomy in the United States is $20.22, according to ZipRecruiter salary data. Most workers in this role earn between $17.31 and $22.84 per hour, depending on experience, location, and employer.

What is a 2nd shift phlebotomy?

A 2nd Shift Phlebotomy job refers to a position where a phlebotomist works during the second shift, typically in the afternoon to late evening. This role involves drawing blood from patients, labeling samples, and ensuring proper handling for laboratory testing. Phlebotomists in this shift often work in hospitals, labs, or clinics, assisting with patient care outside of standard daytime hours. The schedule can vary depending on the facility, but it usually starts in the afternoon and ends at night.

What does a typical shift look like for a 2nd shift phlebotomist?

A 2nd Shift Phlebotomist usually works in clinical settings during late afternoon to evening hours, often from around 2:00 PM to 10:00 PM. During a typical shift, you’ll be responsible for drawing blood from patients, properly labeling and transporting samples, and ensuring all documentation is completed accurately. You may collaborate closely with nurses, lab technicians, and other healthcare staff to manage patient flow and meet specimen collection deadlines. Due to the timing, you might experience a mix of steady periods and busy times, especially as hospital or clinic volumes fluctuate. This shift can offer a supportive team environment with the opportunity for increased independence and responsibility compared to daytime shifts.

What are the key skills and qualifications needed to thrive in the 2nd shift phlebotomy position?

To excel in a 2nd Shift Phlebotomy role, you need proficiency in venipuncture techniques, knowledge of medical terminology, and completion of a phlebotomy certification program. Familiarity with laboratory information systems, specimen labeling protocols, and safety equipment is typically required. Exceptional communication, attention to detail, and the ability to remain calm under pressure are valuable soft skills. These competencies ensure accurate specimen collection, patient comfort, and efficient workflow during evening or nighttime hours.

More about 2Nd Shift Phlebotomy jobs
What are the most commonly searched types of Phlebotomy jobs? The most popular types of Phlebotomy jobs are:
What states have the most 2Nd Shift Phlebotomy jobs? States with the most job openings for 2Nd Shift Phlebotomy jobs include:
Infographic showing various 2Nd Shift Phlebotomy job openings in the United States as of July 2026, with employment types broken down into 3% As Needed, 70% Full Time, 19% Part Time, and 8% Contract. Highlights an 99% Physical, and 1% Remote job distribution, with an average salary of $42,055 per year, or $20.2 per hour.

Inpatient Phlebotomy - Second Shift

Duke Clinical Research Institute

Durham, NC • On-site

$16.25 - $21.50/hr

Other

Posted 8 days ago


Job description

At Duke Health, we're driven by a commitment to compassionate care that changes the lives of patients, their loved ones, and the greater community. No matter where your talents lie, join us and discover how we can advance health together.

Duke University Health System Clinical Laboratories

Pursue your passion for caring and innovation as a team member in the state-of-the-art Duke Health laboratories. Duke Health laboratories are College of American Pathologists accredited and Clinical Laboratory Improvement Amendments certified. The multidisciplinary team is committed to serving the rare and complex testing needs of Duke Health patients by providing timely and accurate results, educating the next generation of laboratorians, scientists and physicians, and researching and developing the cutting-edge diagnostics of tomorrow.

Inpatient phlebotomy at Duke Regional Hospital in Durham. Monday - Friday, 12:30 p.m. - 9:00 p.m. and one in three weekends.

This position is eligible for the second shift differential.

General Description

Perform a variety of the technical procedures involved in the procurement, processing, and evaluation of patient specimens for use in the diagnosis and treatment of disease; teach and perform routine clerical and technical procedures including specimen collection, and responsible for the delivery of supplies and procurement of specimens from physicians' offices, clinical specimen preparation and processing for analysis and dispatch, patient registration and order entry; and serve as hospital laboratory representative and liaison with outside clients.

Job Duties

  • Perform a variety of routine and specialized procedures.
  • Prepare and initiate special tests and procedures including, but not limited to, cytogenetics, general laboratory, Point-of-Care Testing, urinalysis without a microscope, phlebotomy, and specimen management.
  • Obtain total volume, perform aliquot and distribute all urine specimens.
  • Properly determine urine volume. Properly label aliquot containers.
  • Communicate in a professional and courteous manner with patients, family, and other medical personnel as necessary for problem recognition and initiation of the resolution and, if necessary, escalation to the next level. Obtain information for laboratory records, explain procedures, allay fears and elicit cooperation.
  • Utilize standard equipment to maintain positive patient identification through the maintenance of all clinical laboratory records, register/accession patients/specimens, and perform order entry as required.
  • Perform various quality control, safety, and infection control procedures to maintain compliance with internal and external regulations.
  • Receive and process specimens as required by respective laboratory area procedures and protocols.
  • Receive specimens into the laboratory using Beaker.
  • Positively ID all patient specimens, and verify all laboratory labels/requisitions, ensuring a valid order. Properly sort all specimens.
  • Participate in the distribution of specimens, supplies, and reports to the appropriate laboratory staff; perform messenger/courier activities as required.
  • Maintain inventory of supplies; stock supplies on carts and hand trays; wash glassware.
  • Utilize various hospital information systems and software.
  • Assist with the maintenance of specimens and/or slides.
  • Perform routine maintenance as required by laboratory procedures.
  • Assist with word processing, miscellaneous office tasks, and maintaining office records.
  • Perform other related duties incidental to the work described herein.

Education

High School diploma or GED. For assignment to phlebotomy, completion of a structured phlebotomy training course and clinical rotation is recommended.

Experience

  • Alternatively, an equivalent combination of relevant education and/or experience.
  • Must have at least 2 years experience as a Clinical Tech I or Certification as a Phlebotomist plus achieve the required competency level for specified job responsibilities.

Degrees, Licensure, and/or Certification

  • For assignment to Phlebotomy Services, certification as a Phlebotomist by a nationally recognized board/agency may be required.
  • Certification of Basic Life Support preferred.
  • Pediatric Phlebotomy experience required.
  • National Healthcare Association (NHA) --Clinical Laboratory Phlebotomy Examination (CLPlb), American Society of Clinical Pathologists-- Phlebotomy Technician Examination [PBT(ASCP)], International Academy of Phlebotomy Sciences (IAPS), National Phlebotomy Association (NPA), American Society of Phlebotomy Technicians (ASPT), National Phlebotomy Certification Examination (NPCE), National Phlebotomy Solutions (NPS), American Medical Technologists (AMT), or another national certification as approved by Clinical Labs leadership.

Knowledge, Skills, and Abilities

  • Basic phlebotomy and specimen processing techniques.
  • Knowledge of medical terms preferred.
  • Basic computer skills.
  • Effective communication skills.
  • Effective professional communication skills to interact with patients/ customers.

Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.


Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.


Essential Physical Job Functions:

Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.