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Third Shift Lab Assistant Jobs (NOW HIRING)

RemX

Pathology Lab Assistant

Pleasant Prairie, WI · On-site

$20 - $26/hr

Lab Assistant I (3rd Shift - Full-Time) Sun, Mon-Thurs | 8:00 PM - 4:30 AM * Lab Assistant I (Part-Time) Mon-Fri | 4:00 PM - 8:00 PM (20 hours/week) * Pathology Lab Assistant II (2nd Shift ...

Pride Health

Lab Assistant- 3rd Shift

Miramar, FL · On-site

$19.78/hr

Lab Assistant - 3rd shift Location: Miramar, FL 33025 Shift/Time Zone: Tuesday - Saturday 1:30AM - 10:00AM Pay Rate: $19.78/hr (Offered pay rate will be based on education, experience, and healthcare ...

New

Baptistfirst

The Lab Assistant shall coordinate specimen processing area in order to ensure the collection ... Regular-Full time Shift : 7 on / 7 off - Third Shift (United States of America)

Actalent

3rd Shift Lab Technician

Waukesha, WI

$24 - $28/hr

This is a 3rd shift position in an engineering lab, Sunday - Thursday, 11pm - 8am. The lab is set ... Assist with the assembly and fabrication of test equipment and prototype products according to ...

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How much do third shift lab assistant jobs pay per hour?

As of Jun 27, 2026, the average hourly pay for third shift lab assistant in the United States is $20.25, according to ZipRecruiter salary data. Most workers in this role earn between $16.59 and $23.08 per hour, depending on experience, location, and employer.

What are the key skills and qualifications needed to thrive as a Third Shift Lab Assistant, and why are they important?

To thrive as a Third Shift Lab Assistant, you need basic laboratory skills, attention to detail, and at least a high school diploma or equivalent, with some employers preferring additional coursework in science. Familiarity with laboratory information systems (LIS), specimen processing equipment, and adherence to safety protocols is typically required. Strong organizational skills, reliability, and effective communication are important soft skills, especially when working independently overnight. These abilities ensure accurate, timely sample processing and maintain lab workflow during off-peak hours, supporting overall patient care.

What are some common challenges faced by Third Shift Lab Assistants, and how can they be managed?

Third Shift Lab Assistants often face challenges such as working overnight hours, which can affect sleep patterns and social life. Additionally, there may be fewer staff members on duty, requiring greater independence and problem-solving skills. Managing these challenges involves maintaining a consistent sleep schedule, practicing effective communication with day shift colleagues during handovers, and staying organized to ensure accurate documentation and sample processing. Employers typically provide training and support to help new hires adjust to the unique demands of the third shift.

What is the difference between Third Shift Lab Assistant vs Second Shift Lab Assistant?

AspectThird Shift Lab AssistantSecond Shift Lab Assistant
Work Hours10 PM - 6 AM2 PM - 10 PM
CertificationsHigh school diploma, relevant lab experienceHigh school diploma, relevant lab experience
Work EnvironmentLaboratory, manufacturing, or research facilitiesLaboratory, manufacturing, or research facilities
Industry UsageCommon in manufacturing, pharmaceuticals, biotech

The main difference between a Third Shift Lab Assistant and a Second Shift Lab Assistant is their working hours. Both roles typically require similar credentials and work in the same environments within industries like pharmaceuticals and biotech. The shift timing impacts work-life balance and scheduling but does not significantly change job responsibilities or qualifications.

What are Third Shift Lab Assistants?

Third Shift Lab Assistants are laboratory professionals who work overnight or late evening hours, typically covering the third shift to ensure that lab operations continue around the clock. Their responsibilities often include preparing specimens, running tests, maintaining equipment, and accurately recording data during hours when regular staff are not present. This role is crucial in hospitals and research facilities that require 24/7 lab services. Third shift workers may also assist with urgent cases and help streamline workflow for the day shift. Working these hours often comes with shift differentials and unique challenges, such as maintaining alertness and adjusting to an atypical sleep schedule.
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What cities are hiring for Third Shift Lab Assistant jobs? Cities with the most Third Shift Lab Assistant job openings:
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Third Shift Lab Assistant jobs near you
Infographic showing various Third Shift Lab Assistant job openings in the United States as of June 2026, with employment types broken down into 67% Full Time, 8% Part Time, and 25% Contract. Highlights an 91% Physical, 3% Hybrid, and 6% Remote job distribution, with an average salary of $42,113 per year, or $20.2 per hour.
Pathology Lab Assistant

Pathology Lab Assistant

RemX

Pleasant Prairie, WI • On-site

$20 - $26/hr

Full-time

Medical, Dental, Vision, Life, Retirement, PTO

Posted 19 days ago

Job description

Pathology Lab Assistant I & II (Multiple Openings)

Location: Pleasant Prairie, WI
Department: Pathology / Laboratory Services
Employment Type: Full-Time & Part-Time Opportunities Available

Open Positions & Shifts

  • Lab Assistant I (3rd Shift – Full-Time)
    Sun, Mon–Thurs | 8:00 PM – 4:30 AM
  • Lab Assistant I (Part-Time)
    Mon–Fri | 4:00 PM – 8:00 PM (20 hours/week)
  • Pathology Lab Assistant II (2nd Shift – Full-Time)
    Mon–Fri | 3:00 PM – 11:30 PM

Compensation

  • PLA I: $20.00 – $21.00/hour (High school diploma required)
  • PLA II: $21.00 – $22.00/hour (Science degree required)


Shift Differentials:

  • 2nd Shift: +$2.00/hour
  • 3rd Shift: +$4.00/hour


Position Summary

The Pathology Lab Assistant I & II supports laboratory operations by performing specimen handling, data entry, and clerical functions to ensure accurate and timely processing of patient samples. This role is essential in maintaining workflow efficiency and supporting diagnostic services.


Key Responsibilities

Lab Assistant I:

  • Receive, label, and prepare specimens for processing
  • Enter patient and specimen data into laboratory systems
  • Maintain specimen integrity and follow proper handling procedures
  • Assist with clerical and administrative lab tasks
  • Ensure compliance with safety and quality standards

Lab Assistant II (Advanced Responsibilities):

  • Perform all Lab Assistant I duties
  • Verify and reconcile specimen information for accuracy
  • Assist with more complex specimen processing and workflow coordination
  • Troubleshoot specimen discrepancies
  • Support training of new or junior staff


Qualifications

Lab Assistant I:

  • High school diploma or equivalent
  • 0–2 years of experience in healthcare, laboratory, or customer service
  • Strong attention to detail and organizational skills
  • Basic computer/data entry skills

Lab Assistant II:

  • Associate’s or Bachelor’s degree in a science-related field required
  • 2+ years of laboratory or healthcare experience preferred
  • Knowledge of medical terminology and specimen processing
  • Strong understanding of lab workflow and compliance standards


Skills & Competencies

  • High attention to detail and accuracy
  • Ability to work in a fast-paced, high-volume environment
  • Strong communication and teamwork skills
  • Effective time management and prioritization
  • Familiarity with EMR/LIS systems (preferred)


Why Join Us?

  • Competitive pay with shift differentials
  • Multiple scheduling options for flexibility
  • Opportunity for growth within laboratory services
  • Stable healthcare environment with hands-on experience in pathology
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About RemX

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RemX is a proven leader in the Contract to Hire job industry. We help place the right people in the right jobs. Let us help you today!

Industry

Recruiting and staffing services

Company size

501 - 1,000 Employees

Headquarters location

Atlanta, GA, US

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