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Third Shift Lab Assistant Jobs (NOW HIRING)

RemX

Pathology Lab Assistant

Pleasant Prairie, WI · On-site

$20 - $26/hr

Lab Assistant I (3rd Shift - Full-Time) Sun, Mon-Thurs | 8:00 PM - 4:30 AM * Lab Assistant I (Part-Time) Mon-Fri | 4:00 PM - 8:00 PM (20 hours/week) * Pathology Lab Assistant II (2nd Shift ...

Sarahbush

$16.94/hr

Lab Assistant-Phlebotomy (3rd Shift) Lab Assistants work in Phlebotomy, Outreach, and/or Processing and are responsible for duties that may include some or all of the following: patient intake ...

Great Lakes Cheese

3rd Shift Lab Technician

Fillmore, UT · On-site

$18 - $24/hr

Work is performed in a lab setting. Occasional exposure to industrial operations including: temperature extremes from approximately 35F to 100F; noise levels in excess of 85 dB; moisture and moving ...

Great Lakes Cheese

3rd Shift Lab Technician

Fillmore, UT · On-site

$18 - $24/hr

... lab setting. • Occasional exposure to industrial operations including: temperature extremes from approximately 35ºF to 100ºF; noise levels in excess of 85 dB; moisture and moving machinery. • ...

PRIDE Health

Laboratory Assistant

Gaithersburg, MD · On-site

$22 - $25/hr

Pride Health is hiring a Lab Assistant - 3rd shift to support our client's medical facility based in Gaithersburg MD 20877. This is a 3-month assignment with the possibility of a contract-to-hire ...

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How much do third shift lab assistant jobs pay per hour?

As of Jun 4, 2026, the average hourly pay for third shift lab assistant in the United States is $20.25, according to ZipRecruiter salary data. Most workers in this role earn between $16.59 and $23.08 per hour, depending on experience, location, and employer.

What are the key skills and qualifications needed to thrive as a Third Shift Lab Assistant, and why are they important?

To thrive as a Third Shift Lab Assistant, you need basic laboratory skills, attention to detail, and at least a high school diploma or equivalent, with some employers preferring additional coursework in science. Familiarity with laboratory information systems (LIS), specimen processing equipment, and adherence to safety protocols is typically required. Strong organizational skills, reliability, and effective communication are important soft skills, especially when working independently overnight. These abilities ensure accurate, timely sample processing and maintain lab workflow during off-peak hours, supporting overall patient care.

What are some common challenges faced by Third Shift Lab Assistants, and how can they be managed?

Third Shift Lab Assistants often face challenges such as working overnight hours, which can affect sleep patterns and social life. Additionally, there may be fewer staff members on duty, requiring greater independence and problem-solving skills. Managing these challenges involves maintaining a consistent sleep schedule, practicing effective communication with day shift colleagues during handovers, and staying organized to ensure accurate documentation and sample processing. Employers typically provide training and support to help new hires adjust to the unique demands of the third shift.

What are Third Shift Lab Assistants?

Third Shift Lab Assistants are laboratory professionals who work overnight or late evening hours, typically covering the third shift to ensure that lab operations continue around the clock. Their responsibilities often include preparing specimens, running tests, maintaining equipment, and accurately recording data during hours when regular staff are not present. This role is crucial in hospitals and research facilities that require 24/7 lab services. Third shift workers may also assist with urgent cases and help streamline workflow for the day shift. Working these hours often comes with shift differentials and unique challenges, such as maintaining alertness and adjusting to an atypical sleep schedule.

What is the difference between Third Shift Lab Assistant vs Second Shift Lab Assistant?

AspectThird Shift Lab AssistantSecond Shift Lab Assistant
Work Hours10 PM - 6 AM2 PM - 10 PM
CertificationsHigh school diploma, relevant lab experienceHigh school diploma, relevant lab experience
Work EnvironmentLaboratory, manufacturing, or research facilitiesLaboratory, manufacturing, or research facilities
Industry UsageCommon in manufacturing, pharmaceuticals, biotech

The main difference between a Third Shift Lab Assistant and a Second Shift Lab Assistant is their working hours. Both roles typically require similar credentials and work in the same environments within industries like pharmaceuticals and biotech. The shift timing impacts work-life balance and scheduling but does not significantly change job responsibilities or qualifications.

More about Third Shift Lab Assistant jobs
What cities are hiring for Third Shift Lab Assistant jobs? Cities with the most Third Shift Lab Assistant job openings:
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What job categories do people searching Third Shift Lab Assistant jobs look for? The top searched job categories for Third Shift Lab Assistant jobs are:
Third Shift Lab Assistant jobs near you
Infographic showing various Third Shift Lab Assistant job openings in the United States as of May 2026, with employment types broken down into 67% Part Time, and 33% Nights. Highlights an 100% In-person job distribution, with an average salary of $42,113 per year, or $20.2 per hour.
Lab Assistant-Phlebotomy (3rd Shift)

Lab Assistant-Phlebotomy (3rd Shift)

Sarah Bush Lincoln

Mattoon, IL • On-site

$16.94/hr

Part-time

Posted 10 days ago

Sarah Bush Lincoln rating

7.6

Company rating: 7.6 out of 10

Based on 42 frontline employees who took The Breakroom Quiz

184th of 865 rated healthcare providers

Job description

Internal Employees: Please ensure that you are logged into Workday and applying through the Jobs Hub before proceeding.
Lab Assistant-Phlebotomy (3rd Shift)
Job Description
Lab Assistants work in Phlebotomy, Outreach, and/or Processing and are responsible for duties that may include some or all of the following: patient intake (customer service, reception, registration, scheduling), order management (activating / entering orders or following up with provider offices to clarify or complete information), specimen collection (phlebotomy techniques, collection requirements, and specimen handling), specimen flow (transport, receipt, processing, distribution to testing areas, tracking, storage, retrieval, and disposal), point-of-care testing, and assisting scientists, technologists or technicians in clinical areas as directed.
Department: Lab
Hours: Part-Time, 20 hours a week required
Shift: 3rd Shift
Required: High School Diploma, BLS within 90 days of hire
Pay: Based on experience, starting at $16.94 an hour
$500.00 Sign on Bonus
Responsibilities
Assists in putting away supplies delivered to work areas by Materials Management., Collects throat, nasal, and nasopharyngeal swabs using appropriate procedures and supplies., Collects urine and/or alcohol for non-chain-of-custody drug screen testing., Communicates with other shifts about pending specimens needing collected., Evaluates orders to perform appropriate specimen collection per providers' orders., Maintains work areas in a neat and orderly fashion; cleans, decontaminates counters and drawing stations on every shift, and empties sharps disposal containers., Other duties as assigned., Performs phlebotomy techniques - via venipuncture and capillary puncture - using appropriate procedures and supplies including site selection, order of draw, and test requirements., Prioritizes draws by date and time of specimen collection., Researches and assists with problem-solving regarding specimens and lab results., Routinely prints unreceived specimen collection lists and handles any unresolved specimen issues., Takes inventory and re-stocks phlebotomy trays, chairs and cabinets, and lists the supplies that need to be reordered., Utilizes safety tools for patient identification and specimen labeling, including handheld phlebotomy scanners and/or verbal interaction with patients to validate and verify accuracy of information.
Requirements
High School (Required)BLS or CPR certification (within 90 days of hire) - Sarah Bush Lincoln
Compensation
Estimated Compensation Range
$16.94 - $26.26
Pay based on experience

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