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About the Job
This research position is within the Translational Center for Resuscitative Trauma Care, in the vascular biology laboratory of Dr. Eric Wise. Our mission is to develop and evaluate innovative,
effective acute trauma care from point of injury through early post-injury in austere, low resource, rural, and emergency care settings. We also work in understanding the physiology and mechanisms of sepsis in the vasculature. This role is highly hand-on and involves coordinating complex large-animal surgical experiments, managing tissues in real time, and
balancing wet lab assays with post experimental data analysis. Create a multidisciplinary, translational research center that brings together trauma and emergency care research across the state of Minnesota and develops faculty and trainee research careers.
This position supports pre-clinical research within the Center, funded by the Department of Defense. Successful candidates must be able to independently execute multi-step experimental protocols, anticipate challenges, and troubleshoot in real time with minimal supervision.
Responsibilities:
Experimental Research (70%)
The research program employs an integrated, translational experimental approach utilizing large animal (porcine) models, rodent models, and cell culture systems, combined with muscle bath, genomic, proteomic, and metabolomic technologies to answer lab-specific questions related to vascular biology, trauma physiology, and sepsis mechanisms.
Large Animal (Porcine) Studies
Extensive involvement in large animal (porcine) surgical procedures in an operating room setting, including long, time-sensitive experimental days
Manage large animal procedures, including perioperative support, drug administration, and coordination with surgical teams in compliance with IACUC protocols
Collect and analyze tissue, blood, and organ samples for downstream molecular and functional analysis
Rodent Model Studies
Conduct rodent surgical procedures and experimental protocols
Manage small animal (rodent) procedures, including perioperative care and drug administration in compliance with IACUC protocols
Collect and analyze pre-clinical data from rodent models to validate and complement large animal findings
Vascular Reactivity and Muscle Bath Systems
Maintain and utilize muscle/organ bath systems for vascular reactivity studies across human samples large animal, rodent, and isolated vessel preparations Cell Culture, and Molecular Analysis
Perform cell culture and molecular and tissue analysis to assess vascular and inflammatory responses, including proteomic analysis, genomic analysis, imunohistochemistry (IHC) and
Metabolomic sample preparation
Conduct experiments using animal and human samples and associated data.
Collaboration, Model Development, and Mentorship
Collaborate with the Experimental Surgery teams
Troubleshoot technical and experimental issues in real time
Evaluate and optimize new and developing experimental models.
Able to work in team setup as well as Independently.
Train and mentor graduate and medical students, ensuring adherence to protocols and
safety standards
Occasional overnight experiments depending on study design
Data Analysis and Management (25%)Â
Perform data entry; manage and maintain detailed records of experiments and procedures
Perform basic statistical analysis of data using available software
Generate and disseminate experiment reports to research teams and collaborators
Manage sample repository, ensuring proper labeling, storage, and tracking of biological
specimens and lab data
Additional duties (5%)Â
Works under general supervision with ongoing R&D operations as time allows
Other duties as required
Potential visits to animal lab at USUHS (Washington, DC) and/ or Vanderbilt University
(Nashville, TN) for training and collaborative studies
Participate and Present in Seminars and Nationally Renowned Conferences