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Night Shift Phlebotomy Jobs (NOW HIRING)

Phlebotomy Workflow Coordinator

Dayton, OH

$17.50 - $23/hr

Located at Miami Valley Hospital (Dayton, OH) Full-Time Night Shift (11:00 PM-7:30 AM) Night Shift is Eligible for a 15% Shift Differential. Must Have 1 Year of Phlebotomy Experience. Position ...

Phlebotomist Night Shift

Janesville, WI · On-site

$16.75 - $21/hr

Phlebotomist Night Shift Job Location: Janesville, WI Job Type: Contract * Proficient with Hospital ... Proficiently perform phlebotomy on patient population to obtain blood specimens. * Possess the ...

Phlebotomist - Night shift

Rome, GA · On-site

$20.80 - $31.20/hr

Keeps the phlebotomy area clean and stocked with supplies. * Follows state, OSHA and other agency ... Premium pay such as shift, on call, and more based on a teammate's job * Incentive pay for select ...

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Night Shift Phlebotomy information

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How much do night shift phlebotomy jobs pay per hour?

As of Aug 15, 2026, the average hourly pay for night shift phlebotomy in the United States is $20.22, according to ZipRecruiter salary data. Most workers in this role earn between $17.31 and $22.84 per hour, depending on experience, location, and employer.

What is the difference between Night Shift Phlebotomy vs Night Shift Medical Assistant?

AspectNight Shift PhlebotomyNight Shift Medical Assistant
CertificationsPhlebotomy certificationCPR, Medical Assistant certification
Work EnvironmentHospitals, clinics, blood banksClinics, hospitals, outpatient facilities
Job ResponsibilitiesBlood draws, specimen collectionVitals, patient prep, basic clinical tasks
Industry UsageHealthcare, laboratory servicesPrimary care, outpatient care

Night Shift Phlebotomy and Night Shift Medical Assistant roles share healthcare settings and some certifications but differ mainly in responsibilities. Phlebotomists focus on blood collection, while Medical Assistants handle broader clinical tasks. Both roles are essential in patient care during night shifts.

What skills and qualifications are needed to thrive as a night shift phlebotomist?

To thrive as a Night Shift Phlebotomist, you need a solid understanding of venipuncture techniques, specimen handling, and infection control protocols, usually supported by a phlebotomy certification or relevant training. Familiarity with laboratory information systems (LIS), barcode scanners, and electronic medical records is typically required. Strong attention to detail, effective communication, and the ability to work independently during overnight hours are essential soft skills. These abilities ensure accurate, safe specimen collection and patient care in a setting that demands both technical precision and adaptability.

What unique challenges might I face working as a night shift phlebotomist, and how can I prepare for them?

Night shift phlebotomists often work with limited staff and may encounter patients who are asleep or less responsive, requiring extra care when drawing blood. You'll also need to adapt to the hospital's quieter atmosphere and handle urgent, time-sensitive requests, especially in emergency situations. Building strong communication with the night nursing team and developing excellent organizational skills will help you succeed. It's important to prioritize self-care and establish a healthy sleep routine to manage the demands of overnight work.

What is a night shift phlebotomist?

Night shift phlebotomists are medical professionals who specialize in drawing blood from patients during nighttime hours, typically in hospitals, clinics, or laboratories that operate 24/7. Their primary responsibilities include collecting blood samples, labeling specimens accurately, and ensuring proper handling for laboratory testing. Working at night, they often care for patients with urgent needs or those admitted outside of regular hours. Night shift phlebotomists play a vital role in supporting timely diagnosis and treatment by ensuring that blood samples are collected and processed efficiently, regardless of the time of day.
More about Night Shift Phlebotomy jobs

What cities are hiring for Night Shift Phlebotomy jobs?

Cities with the most Night Shift Phlebotomy job openings:

What states have the most Night Shift Phlebotomy jobs?

States with the most job openings for Night Shift Phlebotomy jobs include:

Infographic showing various Night Shift Phlebotomy job openings in the United States as of August 2026, with employment types broken down into 1% As Needed, 72% Full Time, 21% Part Time, 4% Contract, and 2% Nights. Highlights an 99% Physical, and 1% Remote job distribution, with an average salary of $42,055 per year, or $20.2 per hour.

Phlebotomist/Lab Assistant -Full-Time Night Shift

Three Crosses Regional Hospital

Las Cruces, NM • On-site

$16.75 - $21/hr

Full-time

Posted 15 days ago


Job description

Phlebotomist/Lab Assistant -Full-Time Night Shift

If you're looking for a place to call home and grow, Three Crosses Regional Hospital is looking for you! We are looking for a Full-Time night shift Phlebotomist/Lab Assistant that is committed to clinical excellence and building a patient-centered culture.

Three Crosses Regional Hospital is an advanced independent healthcare organization led by a local team of professionals dedicated to high-quality patient care and being the first choice of patients and providers in the communities we serve.

We are dedicated to hiring team members that will adapt to our culture, pride themselves in professionalism, integrity, transparency, two-way communication, and ensuring the safety and well-being of our patients and staff.

Under the general supervision of the Laboratory Director and MT/MLT’s, the Phlebotomist/Laboratory Assistant is responsible for efficiently and accurately performing blood draws, waived testing, clerical functions, and specimen processing that will include accessioning of specimens submitted to the laboratory as well as specimens collected by other staff/physician offices/clinics. Will provide patient care in accordance with TCRH policies and procedures. Expected to provide a positive image of TCRH and to be able to communicate effectively with patients, peers, physicians and visitors.

The incumbent will carry out duties by adhering to the highest standards of ethical and moral conduct, acts in the best interest of Three Crosses Regional Hospital, and fully supports the mission, vision, and values of Three Crosses Regional Hospital.

Primary Responsibilities

  • Skilled in laboratory techniques to encompass all age ranges.
  • Basic clerical skills, phone etiquette. Interdisciplinary collaboration and communication.
  • Ability to communicate and interact with patients in a manner that makes them feel comfortable with the laboratory assistant’s ability to perform procedure.
  • Must be able to understand the importance of specimen handling from the delivery to the department, entry into the computer system, to the final report.
  • Understands the importance of making sure every detail is met for each sample is unique.
  • There are specimens in the laboratory that are difficult to recollect (i.e. CSF, Bone Marrow, etc.)
  • Must participate within a team-centered approach toward patient care.
  • Must observe professional ethics in maintaining confidential information acquired regarding personal, financial, medical, or employment of patients, families, and employees of TCRH.
  • Demonstrate critical thinking, time management and problem-solving skills.
  • Excellent verbal and written communication (including documentation) skills.
  • Excellent relationship management with patients, families, and care providers.
  • Ability to organize and prioritize tasks.
  • Follow and enforce safety practices for patients and TCRH.
  • Attend staff meetings as needed.
  • Other duties as assigned.

Education and Experience

  • Required: High School Diploma or equivalent
  • Required: Graduate of a phlebotomy program or 2 years equivalent experience
  • Required: 1-2 years of experience working in a high-volume laboratory in the role of a phlebotomist.
  • Preferred: Hospital setting experience.
  • Required: Meet all Employee Health Requirement
  • Required: BLS certification from AHA
  • Preferred: Certification as Certified Phlebotomy Technician (CPT), Phlebotomy Technician, PBT (ASCP)

We are an equal opportunity employer, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity or expression, pregnancy, age, national origin, disability status, genetic information, protected veteran status, or any other characteristic protected by law”.