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In Vitro Assay Jobs (NOW HIRING)

Astrix Inc

In Vitro Scientist III

Ridgefield, CT · On-site

$49 - $54/hr

Design, develop, validate, and execute in vitro assays to study lymphocyte (B cell and T cell) function and modulation * Conduct experiments supporting target validation, compound profiling, and hit ...

Crown Bioscience, Inc.

This role oversees global assay platforms, multi-site scientific teams, operational strategy, and capability expansion across cell-based, immunology, imaging-based, and translational in vitro systems.

CYNET SYSTEMS

Scientist III

Ridgefield, CT · On-site

$49/hr

This position involves conducting routine and non-routine cellular assays to support in vitro assay development. Requirement/Must Have: * Master's degree from an accredited institution with one-plus ...

Cogent Scientific

Expert In Vitro Biologist

Manhattan, NY · On-site

$110K - $125K/yr

Establish and optimize novel in vitro assays using 3D organotypic models, complex co-cultures, organoids, transwell systems, air-liquid interface, and/or organ-on-a-chip platforms representing both ...

Eurofins

In Vitro Scientist

New Brunswick, NJ · On-site

$25 - $35/hr

Creates and maintains lab assay schedule. * Leads training of In Vitro Scientists. * Other duties as assigned. Qualifications * B.S. degree or equivalent experience in laboratory animal medicine ...

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How much do in vitro assay jobs pay per hour?

As of Sep 14, 2026, the average hourly pay for in vitro assay in the United States is $45.04, according to ZipRecruiter salary data. Most workers in this role earn between $37.98 and $50.48 per hour, depending on experience, location, and employer.

What is an in vitro assay?

An in vitro assay is a laboratory technique used to study biological or chemical processes outside of a living organism, typically in a controlled environment such as test tubes or petri dishes. These assays allow researchers to analyze the effects of drugs, chemicals, or biological agents on cells, tissues, or biomolecules. In vitro assays are widely used in drug discovery, toxicology, and biomedical research because they offer a faster, more controlled, and ethical alternative to in vivo (within the living organism) experiments. The results help scientists understand mechanisms of action, efficacy, and safety before proceeding to animal or clinical studies.

What are the key skills and qualifications needed to thrive as an in vitro assay scientist?

To thrive as an In Vitro Assay Scientist, you need a strong background in biology, chemistry, or a related field, typically with a bachelor's or advanced degree and laboratory experience. Familiarity with laboratory instrumentation, cell culture techniques, and data analysis software like GraphPad Prism or LIMS are essential, along with knowledge of GLP or GMP standards. Attention to detail, problem-solving, and strong communication skills help you accurately conduct experiments and collaborate with research teams. These competencies are crucial for generating reliable data, ensuring compliance, and driving successful research outcomes.

What are some common challenges faced by professionals working in in vitro assay roles, and how can they be addressed?

Professionals working in In Vitro Assay roles often encounter challenges such as ensuring assay reproducibility, troubleshooting unexpected experimental results, and managing tight deadlines in a fast-paced laboratory environment. Attention to detail and strong documentation skills are essential for maintaining data integrity. Collaborating closely with cross-functional teams, such as analytical chemists and research scientists, helps streamline troubleshooting and optimize protocols. Regular training and staying updated with new assay technologies can also help overcome technical hurdles and foster continuous improvement.

What is the difference between In Vitro Assay vs Laboratory Technician?

AspectIn Vitro AssayLaboratory Technician
Required CredentialsBachelor's degree in biology, biochemistry, or related field; laboratory skillsAssociate's or bachelor's degree; basic lab skills
Work EnvironmentResearch labs, pharmaceutical companies, biotech firmsHospitals, research labs, diagnostic labs
Job FocusConducting specific in vitro experiments to test biological activitySupporting lab operations, preparing samples, maintaining equipment

In Vitro Assay specialists focus on designing and performing specific laboratory tests to analyze biological interactions, often requiring advanced knowledge of assay techniques. Laboratory Technicians support overall lab functions, handling sample preparation and equipment maintenance. While both roles work in laboratory settings and require similar educational backgrounds, In Vitro Assay professionals typically engage in more specialized testing and experimental design.

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In Vitro Assay jobs near you
Infographic showing various In Vitro Assay job openings in the United States as of July 2026, with employment types broken down into 76% Locum Tenens, 17% Full Time, 1% Contract, and 6% Summer. Highlights an 93% Physical, 2% Hybrid, and 5% Remote job distribution, with an average salary of $93,688 per year, or $45 per hour.

Scientist II, In-Vitro & Nonclinical Assay Development

Frederick, MD • On-site

Tonix Pharmaceuticals
Pharmaceutical and Medicine Manufacturing • 51 - 200 employees
Learn more about Tonix Pharmaceuticalsexternal

Full-time

Posted 24 days ago

Job description

Job Summary

Scientist II will support Tonix Pharmaceuticals' preclinical research programs through the development, optimization, and execution of in vitro and nonclinical assays. The role will contribute to translational research using cell-based assays, DMPK/ADME assays, 3D cell culture and organoid systems, and other nonclinical models to evaluate therapeutic candidates. The scientist will work collaboratively across discovery and development programs and generate high-quality, reproducible data to support program decisions.

Essential Functions

Develop, optimize, and execute in vitro cell-based and biochemical assays to support drug discovery and preclinical development programs.

Perform and support DMPK/ADME assays, including permeability, protein binding, metabolic or CYP-related assays, cytotoxicity, and other screening assays as appropriate.

Develop and evaluate 3D cell culture, spheroid, and organoid-based assay systems for translational and nonclinical studies.

Support the development and execution of nonclinical assays and models for evaluation of therapeutic efficacy, pharmacology, safety, and mechanism of action.

Design experiments, develop and optimize protocols, analyze data, troubleshoot assays, and document results in laboratory records and technical reports.

Maintain mammalian cell culture systems, including established cell lines and primary or specialized cell systems as needed for project-specific assays.

Apply molecular, biochemical, imaging, and viability-based assay methods, including techniques such as RT-PCR, Western blotting, ELISA, luminescence-based assays, microscopy, and related analytical readouts.

Work collaboratively with internal scientists and external partners/CROs to support preclinical and DMPK studies.

Prepare data summaries, presentations, protocols, reports, and other documentation supporting research programs and regulatory or development activities.

Maintain accurate records and perform work in accordance with applicable laboratory, quality, and safety procedures.

Necessary Skills and Abilities

Strong hands-on experience with mammalian cell culture and in vitro assay development.

Experience designing, optimizing, troubleshooting, and interpreting cell-based, biochemical, or translational assays.

Familiarity with DMPK/ADME assay concepts and laboratory methods is preferred.

Experience with 3D culture systems, spheroids, organoids, or other advanced in vitro models is preferred.

Experience supporting nonclinical or preclinical drug development studies.

Proficiency with common molecular and biochemical laboratory techniques and quantitative data analysis.

Strong experimental planning, problem-solving, technical writing, presentation, and communication skills.

Ability to work independently while collaborating effectively in multidisciplinary project teams.

Educational Requirements

  • Ph.D. in Pharmacology, Biochemistry, Biology, Biotechnology, Pharmaceutical Sciences, or a related life sciences discipline; with 2+ years of relevant experience or an M.S. with 4+ years of relevant research experience.

Experience Requirements

Relevant hands-on research experience in pharmaceutical, biotechnology, CRO, government, or academic research environments.

Experience with in vitro pharmacology, assay development, DMPK/ADME, 3D culture/organoid models, or nonclinical research is preferred.

Experience generating reproducible experimental data and communicating results to multidisciplinary teams.

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