Senior Next Generation Sequencing Jobs (NOW HIRING)

The Applied Genomics, Computation & Translational Core is looking for a Biomedical Technician to join the team! 

Candidates with experience in single-cell and spatial assay technologies are highly desirable. This includes familiarity with techniques such as single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, multiplex imaging, and other high-dimensional molecular profiling platforms. A working knowledge of sample preparation workflows, library preparation, quality control metrics, and data generation associated with next-generation sequencing or imaging-based assays is strongly valued. Experience handling complex biological specimens and maintaining rigorous data integrity within high-throughput experimental environments is also preferred.

The Cedars-Sinai Applied Genomics, Computation & Translational (AGCT) Core is an automated, high-throughput genomics facility equipped with the latest next-generation sequencing technologies. We specialize in single-cell and spatial omics, utilizing platforms including 10x Genomics (Chromium X, Xenium Analyzer, Visium CytAssist), Parse Biosciences, Scale Biosciences, Illumina, Singular Genomics G4X , and Bruker CosMX SMI. We also offer expertise in long-read sequencing via Oxford Nanopore Technologies (PromethION 24, MinION) and high-throughput short-read sequencing on the Illumina NovaSeq X Plus. We provide comprehensive support covering sample preparation, library construction, and diverse sequencing applications including bulk cell RNA-Seq, WGS/WES, ATAC-Seq, and metagenomics. 

The AGCT Core also delivers extensive bioinformatics analysis. Standard pipelines are established for primary data processing across all services, encompassing read alignment, quality control, expression quantification, variant calling, and peak calling. We also provide downstream analysis packages to help analyze these data in the context of the researcher's experimental design. We actively collaborate with investigators to develop sophisticated or custom computational solutions for specific research questions, including advanced analysis for spatial transcriptomics data. Our bioinformatics support extends to results interpretation, generation of publication-ready figures, and drafting manuscript methodology.

To learn more please visit Applied Genomics, Computation & Translational Core | Cedars-Sinai. 

Are you ready to be a part of breakthrough research? 

The AGCT Core Biomedical Technician contributes to the laboratory sciences team of the lab. They work independently as part of a team under direct supervision of a team lead.  The Biomedical Technician is responsible for performing laboratory benchwork strictly according to Standard Operating Procedures with accurate documentation and a theoretical understanding of assays utilized.  They perform quality control on RNA and DNA samples, understanding the nuances of sample quality and qualifications.  The Biomedical Technician contributes to the success of projects by providing good oral and written communication, documentation, and attentiveness to detail. They work with state-of-the-art technologies and instruments, performing next generation sequencing library preparation and sequencing.

Primary Duties and Responsibilities:

• Performs a variety of project-specific process development tasks for multiple clients following standard operating procedures (SOPs).
• Maintains good documentation in laboratory notebooks, data record/transfer, SOPs, and batch records.
• Drafts standard operating procedures and maintains computer database.
• Verifies samples received, logs samples into systems, and may transport to appropriate environment within the facility for storage following SOPs.
• Performs lab maintenance duties, including glassware cleaning and sterilization.
• Maintains lab equipment and related records.
• Prepares cGMP production room, maintains material inventories, and places orders for equipment and supplies.
• Assists in the operation of specialized equipment and machinery.
• Ensures all activities comply with regulatory guidelines and safety standards, as appropriate.

Department Specific Responsibilities:

• Construction of next generation sequencing libraries for bulk cell, single cell, and/or spatial omics using commercially available reagent kits.
• Illumina short-read sequencing and/or Oxford Nanopore Technologies long-read sequencing of NGS libraries.
• DNA/RNA QC via fluorescent quantification and automated electrophoresis.
• Operation of state-of-the-art genomics equipment including NGS sequencers, automated liquid handlers, and tissue imagers, as well as standard laboratory equipment such as thermal cyclers, centrifuges, and qPCR machines.
• Mathematical calculations, including fold dilution, normalization, and conversions.
• Understand, analyze, and interpret DNA/RNA/NGS library/sequencing QC results. 
• Liaise with post-doctoral, doctoral, and staff researchers, clinical investigators, and principal investigators to provide quality customer service.
• Handle multiple demands and/or manage complex and competing priorities, pivoting as needed based on current needs in a dynamic environment.
• Apply advanced knowledge of science/learning/specialized intellectual instructions to analyze, interpret, or make deductions from varying facts or circumstances.
• Provide and/or support a level of work excellence and accuracy; recognize and address flaws or errors that others may overlook.
• Regular oral and written communication with team members and team lead.
• Accurate and detailed electronic documentation of work performed and results obtained.
• Work independently as part of a team under direct supervision of a team lead.
• Observe and comply with all safety standards and procedures.

Required Qualifications:

• Associate Degree or College Diploma in Biology, Biochemistry, or a related scientific or engineering discipline.
• Minimum of one (1) year of hands-on experience in a laboratory or biotechnology environment.
• Demonstrated experience working with biomedical equipment, including routine maintenance, calibration, troubleshooting, and/or performance testing.

Strongly Preferred:

• Experience with single-cell and spatial assay technologies. This includes familiarity with techniques such as single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, multiplex imaging, or other high-dimensional molecular profiling platforms.
• Working knowledge of sample preparation workflows, library preparation, quality control metrics, and data generation associated with next-generation sequencing or imaging-based assays.
• Experience handling complex biological specimens and maintaining data integrity in high-throughput experimental environments.
• Bachelor's Degree in Biology, Biochemistry, or a related science or engineering field.