ZipRecruiter

Log In

1

Protein Expression Purification Jobs (NOW HIRING)

next page

Showing results 1-20

All JobsScience JobsBiology JobsBiologist JobsProtein Expression Purification Jobs

Protein Expression Purification information

See salary details

$17

$33

$51

How much do protein expression purification jobs pay per hour?

As of Aug 29, 2026, the average hourly pay for protein expression purification in the United States is $33.06, according to ZipRecruiter salary data. Most workers in this role earn between $21.63 and $40.87 per hour, depending on experience, location, and employer.

What is protein expression and purification?

Protein expression and purification refers to the process of producing a specific protein in a host organism, such as bacteria, yeast, or mammalian cells, and then isolating that protein from the host cells or culture medium. This process is fundamental in research and biotechnology for studying protein function, structure, and interactions, as well as for manufacturing proteins for therapeutic or industrial use. The workflow typically involves cloning the gene of interest, expressing it in a suitable system, and using various biochemical techniques to purify the target protein to a high degree of purity.

What are the key skills and qualifications needed to thrive as a protein expression purification scientist, and why are they important?

To thrive as a Protein Expression Purification Scientist, you need a solid background in biochemistry or molecular biology, with hands-on experience in recombinant protein expression and purification techniques. Familiarity with tools such as FPLC/HPLC systems, affinity chromatography, and spectrophotometric analysis, along with knowledge of relevant laboratory safety protocols, is typically required. Strong problem-solving abilities, attention to detail, and effective communication skills are essential soft skills for this role. These competencies ensure the efficient production of high-quality proteins for research or therapeutic applications, supporting critical advancements in biotechnology.

What are some common challenges faced in a protein expression purification role and how can they be addressed?

Professionals in Protein Expression Purification often encounter challenges such as low protein yield, protein aggregation, and difficulties in achieving the desired purity. These issues can be addressed by optimizing expression conditions (such as temperature and induction time), testing different host systems, and employing various purification techniques like affinity chromatography or size-exclusion chromatography. Collaborating closely with other team members, such as molecular biologists and analytical chemists, is essential to troubleshoot problems and refine protocols. Staying up-to-date with the latest methodologies and maintaining meticulous records also help ensure successful outcomes.

What is the difference between Protein Expression Purification vs Protein Biochemist?

AspectProtein Expression PurificationProtein Biochemist
Primary FocusIsolating and purifying proteins for research or productionStudying protein structure, function, and interactions
Required SkillsMolecular cloning, chromatography, cell cultureProtein analysis, assays, structural techniques
Work EnvironmentLaboratories, biotech or pharma companiesResearch labs, academic institutions
Common CertificationsBiotech certifications, molecular biology trainingBiochemistry degrees, research experience

While both roles involve working with proteins, Protein Expression Purification focuses on isolating and purifying proteins, often for production or experimental use. Protein Biochemists analyze protein functions and structures, often using purified proteins. Both roles require molecular biology skills and lab experience but serve different purposes within the research and biotech industries.

More about Protein Expression Purification jobs

What cities are hiring for Protein Expression Purification jobs?

Cities with the most Protein Expression Purification job openings:

What states have the most Protein Expression Purification jobs?

States with the most job openings for Protein Expression Purification jobs include:

What job categories do people searching Protein Expression Purification jobs look for?

The top searched job categories for Protein Expression Purification jobs are:

Protein Expression Purification jobs near you
Infographic showing various Protein Expression Purification job openings in the United States as of August 2026, with employment types broken down into 86% Full Time, and 14% Contract. Highlights an 100% In-person job distribution, with an average salary of $68,765 per year, or $33.1 per hour.

Scientist / Protein Purification

Cambridge, MA • On-site

Pioneer Data
See more jobs at Pioneer Dataexternal

Contractor

Posted 15 days ago

Job description

Position Details:
Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for an Scientist to join their expanding team.
 
Job Title: Scientist / Protein Purification
Duration: 24 months contract, extendable up to 48 months
Location: Cambridge, MA
 
Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
 
Job Description:
We are seeking a motivated Bench Scientist to join a biotherapeutics research team in Cambridge, MA.
This is a hands-on laboratory position focused primarily on recombinant protein expression, purification, and characterization to support downstream biotherapeutic research.

Key Responsibilities
Perform routine recombinant protein purification using FPLC/AKTA and related chromatography techniques.
Support the production of recombinant proteins and antigens for downstream biotherapeutic research.
Participate in protein expression workflows and, as needed, molecular biology/DNA-related activities.
Perform protein characterization and assess protein quality, purity, concentration, and suitability for downstream applications.
Work with antigens, antibodies, mAbs, Fabs, and other recombinant proteins.
Perform routine protein labeling and conjugation, including fluorescent and biotin labeling.
Assist with analytical techniques such as SDS-PAGE, Western blotting, and HPLC.
Maintain accurate laboratory records, sample tracking, and experimental documentation.
Manage multiple protein-production and purification requests while meeting project timelines.
Work collaboratively with scientists across the research organization and provide purified proteins for downstream studies.
Maintain a clean, organized, and compliant laboratory environment and follow all required laboratory safety and PPE procedures.

Required Qualifications
Bachelor's degree in Biochemistry, Biology, Chemistry, Molecular Biology, or a related scientific discipline.
At least 1 year of hands-on experience with recombinant protein purification or a closely related protein-science discipline.
Hands-on experience with AKTA/FPLC or comparable chromatography systems.
Understanding of protein purification and characterization principles.
Strong laboratory organization and time-management skills.
Ability to work effectively in a collaborative, fast-paced research environment.

Preferred Qualifications
Master's degree in Biochemistry, Biology, Biophysics, Molecular Biology, or a related field.
Experience with recombinant protein expression.
Experience with protein biophysics and protein characterization.
Experience working with antibodies, mAbs, Fabs, or recombinant antigens.
Background in immunology or biotherapeutic research.