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Overnight Lab Jobs in Chicago, IL (NOW HIRING)

Certified Group

Chemistry Lab Tech II

Aurora, IL

$18.75 - $25/hr

Maintain lab workspaces in a clean and organized manner at all times * Assisting with managing ... Overnight Travel may be required at the discretion of management * Regularly lift and/or move up to ...

Certified Group

Lab Analyst

Aurora, IL

Working knowledge of aseptic sampling technique, automated lab equipment, and acceptable methods ... Overnight Travel is required at the discretion of management * Occasionally lift and/or move up to ...

Certified Group

Micro Lab Assistant

Aurora, IL

Job Summary The Lab Assistant within the Media Department is responsible for preparing, sterilizing ... Overnight travel may be required at the discretion of management. * Must wear appropriate ...

Certified Group

Chemistry Lab Tech II

Aurora, IL · On-site

$20 - $22/hr

Maintain lab workspaces in a clean and organized manner at all times * Assisting with managing ... Overnight Travel may be required at the discretion of management * Regularly lift and/or move up to ...

Certified Group

Lab Analyst

Aurora, IL · On-site

Working knowledge of aseptic sampling technique, automated lab equipment, and acceptable methods ... Overnight Travel is required at the discretion of management * Occasionally lift and/or move up to ...

Certified Group

Lab Analyst

Aurora, IL

Working knowledge of aseptic sampling technique, automated lab equipment, and acceptable methods ... Overnight Travel is required at the discretion of management * Occasionally lift and/or move up to ...

Advocate Illinois Masonic Medical Center

Cath Lab

Chicago, IL · On-site

  • Medical

  • Dental

  • Vision

  • Life

  • Retirement

RN - Cardiac Cath Lab This position involves working in a Cardiac Cath Lab. The facility is a Level ... There are opportunities for scheduled weekend rotations and overnight call shifts. Emergency ...

Certified Group

Micro Lab Assistant

Aurora, IL

Job Summary The Lab Assistant within the Media Department is responsible for preparing, sterilizing ... Overnight travel may be required at the discretion of management. * Must wear appropriate ...

Certified Group

Micro Lab Assistant

Aurora, IL · On-site

Job Summary The Lab Assistant within the Media Department is responsible for preparing, sterilizing ... Overnight travel may be required at the discretion of management. * Must wear appropriate ...

American Traveler

Travel Cardiac Cath Lab RN

Chicago, IL · On-site

  • Medical

  • Dental

  • Vision

  • Life

  • Retirement

Specialty: Cardiac Cath Lab * Discipline: RN * Start Date: 09/08/2026 * Duration: 13 weeks * 40 ... overnight call shifts per 4-week schedule * 30-minute response time required for call with no sleep ...

LanceSoft

Travel Cath Lab Technologist

Chicago, IL · On-site

$2.3K - $3.1K/wk

  • Medical

  • Dental

  • Vision

... overnight call shifts per 4-week schedule ▪ 30-minute response time ▪ No sleep room available o Holiday Expectations: possibility of being on call o Approval of RTO: up to 7 days o Shift times ...

Simple Laboratories, LLC

Medical Lab Scientist

Chicago, IL · On-site

$34/hr

  • Medical

  • Dental

  • Vision

  • Retirement

  • PTO

Overnight: 11:00 PM to 7:30 AM with alternating weekends ESSENTIAL JOB RESPONSIBILITIES ... We have a dedicated and passionate team of lab technicians, phlebotomists, sales, IT and ...

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All JobsLab JobsOvernight Lab JobsOvernight Lab Jobs in Chicago, IL

Overnight Lab information

See Chicago, IL salary details

$9

$26

$46

How much do overnight lab jobs pay per hour?

As of Aug 15, 2026, the average hourly pay for overnight lab in Chicago, IL is $26.01, according to ZipRecruiter salary data. Most workers in this role earn between $19.81 and $28.70 per hour, depending on experience, location, and employer.

What is an overnight lab?

Overnight Lab jobs typically involve working during nighttime hours in laboratory settings. Employees may be responsible for processing samples, conducting experiments, analyzing data, or maintaining lab equipment while most daytime staff are not present. These roles are crucial for labs that operate 24/7, such as medical testing facilities, research institutions, or industrial labs, to ensure continuous workflow and timely results. Working overnight can involve unique challenges, including managing fatigue and adjusting to a non-traditional schedule, but it also often offers higher pay or shift differentials.

What is the difference between Overnight Lab vs Night Lab Technician?

AspectOvernight LabNight Lab Technician
CredentialsHigh school diploma or equivalent; some roles may require certification in lab proceduresHigh school diploma or equivalent; certification may be preferred
Work EnvironmentLaboratory setting, overnight shifts, often in healthcare or research labsLaboratory setting, overnight shifts, typically in healthcare or diagnostic labs
Employer & IndustryHospitals, diagnostic labs, research facilitiesHospitals, diagnostic labs, healthcare facilities
Common Search & ComparisonOvernight Lab vs Night Lab Technician

The main difference between an Overnight Lab and a Night Lab Technician lies in the job scope. An Overnight Lab often refers to the shift timing or the lab's operational hours, while a Night Lab Technician is a specific role responsible for performing lab tests during overnight shifts. Both roles typically require similar credentials and work in comparable environments, primarily in healthcare or research labs operating overnight hours.

What are some common challenges faced when working an overnight lab shift, and how can they be managed?

Overnight lab positions often require adapting to an unconventional sleep schedule, which can impact alertness and overall health. Managing fatigue is crucial; strategies include maintaining a consistent sleep routine, staying hydrated, and taking short, scheduled breaks during shifts. Communication with daytime staff is also important to ensure smooth handovers and continuity of work. Additionally, overnight staff may encounter limited on-site resources, so strong problem-solving skills and self-sufficiency are valuable in this role.

What are the key skills and qualifications needed to thrive as an overnight lab technician?

To thrive as an Overnight Lab Technician, you need a solid understanding of laboratory procedures, safety protocols, and typically an associate degree or higher in a science-related field. Familiarity with lab instruments, data analysis software, and Laboratory Information Management Systems (LIMS) is often required. Attention to detail, effective time management, and strong problem-solving skills are crucial soft skills for this role. These competencies ensure accurate and timely results, maintain lab safety standards, and support continuous lab operations during overnight shifts.

What are the most commonly searched types of Lab jobs in Chicago, IL?

The most popular types of Lab jobs in Chicago, IL are:

What cities near Chicago, IL are hiring for Overnight Lab jobs?

Cities near Chicago, IL with the most Overnight Lab job openings:

Overnight Lab jobs near you
Infographic showing various Overnight Lab job openings in Chicago, IL as of August 2026, with employment types broken down into 1% As Needed, 77% Full Time, 20% Part Time, and 2% Contract. Highlights an 89% Physical, 2% Hybrid, and 9% Remote job distribution, with an average salary of $54,099 per year, or $26 per hour.

Chemistry Lab Tech II

Certified Group

Aurora, IL

$18.75 - $25/hr

Full-time

Re-posted 10 days ago

Job description

JOB SUMMARY

Assist in the chemical testing of food products, ingredients, and other matrices with primary functions in solution and reagent preparation, sample homogenization, proximate analysis chemistry instrument use (balance, oven, furnace, and extractors), chemistry extractions and procedures, and glassware maintenance.

 ESSENTIAL RESPONSIBILITIES

  • Prepare and maintain chemical solutions
  • Apply standard laboratory techniques in daily workload
  • Inventory and report expendable supplies/materials needed for testing requirements
  • Provide routine preventive maintenance by daily cleaning, sanitizing, and trash disposal
  • Independently pursue an assigned series of processes, procedures, or techniques in accordance with FSNS policies/procedures and customer requirements
  • Maintain laboratory conditions, to include equipment, supplies, quality assurance programs, and accurate records throughout all procedures
  • Perform all activities in safe, hygienic, and efficient manner
  • Share information in an open, and timely manner
  • Maintain lab workspaces in a clean and organized manner at all times
  • Assisting with managing priorities and schedule such that individual goals as well as team goals are achieved with encouraged participation in the Food Safety Net Services Team
  • Responsible for the safety of oneself and others working within their area
  • Responsible for the completion of required training


MINIMUM QUALIFICATIONS

  • Bachelor's degree in chemistry or related field
  • Minimum of 2 years of laboratory experience 
  • ELISA experience or related required
  • Training in general laboratory practices preferred
  • Working knowledge of aseptic sampling technique, automated lab equipment, and acceptable methods and procedures
  • Broad knowledge of concepts, practices, and procedures of a food testing laboratory
  • Ability to travel is preferred
  • Or equivalent combination of education and experience

PHYSICAL DEMANDS

  • Dexterity of hands and fingers to operate a computer keyboard, mouse and to handle other communications/computer components
  • Potential exposure to odors, fumes, airborne particles, hazardous chemicals, and microbiological pathogens
  • Noise level varies from quiet to loud
  • Temperature varies from hot to cold
  • Color vision and depth perception
  • Interactive and fast-paced team oriented tasks
  • Overnight Travel may be required at the discretion of management
  • Regularly lift and/or move up to 25 pounds.

Schedule: Tuesday - Saturday 12pm-8:30pm 

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