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Golden Gate Assembly Jobs (NOW HIRING)

Alignerr

Computer Engineering

Chicago, IL · Remote

$35 - $60/hr

Create rigorous, step-by-step technical solutions, including assembly code, hardware description ... Evaluate AI-generated code (C/C++, Verilog, VHDL), logic gate designs, and operating system kernels ...

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How much do golden gate assembly jobs pay per hour?

As of Jun 6, 2026, the average hourly pay for golden gate assembly in the United States is $16.91, according to ZipRecruiter salary data. Most workers in this role earn between $14.42 and $17.55 per hour, depending on experience, location, and employer.

What are the key skills and qualifications needed to thrive as a Molecular Biologist specializing in Golden Gate Assembly, and why are they important?

To excel as a Molecular Biologist working with Golden Gate Assembly, you need a solid background in molecular biology, genetics, and recombinant DNA techniques, often supported by a relevant degree such as biology or biochemistry. Familiarity with technical tools like PCR machines, DNA sequencing platforms, and specialized molecular cloning software is essential, along with experience in using Type IIS restriction enzymes. Strong attention to detail, problem-solving abilities, and effective collaboration skills distinguish top performers in this role. These skills ensure accurate genetic construct design, efficient workflow, and successful experimental outcomes in research and biotechnology applications.

What is Golden Gate Assembly?

Golden Gate Assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single reaction using type IIS restriction enzymes and DNA ligase. This technique is highly efficient and enables the precise and seamless assembly of genetic parts, such as promoters, coding sequences, and terminators, without leaving unwanted sequences at the junctions. It is widely used in synthetic biology and genetic engineering for building complex DNA constructs quickly and accurately.

What is the difference between Golden Gate Assembly vs Cloning Technician?

AspectGolden Gate AssemblyCloning Technician
CredentialsTypically requires molecular biology or biotechnology degreesRequires similar degrees or certifications in molecular biology or lab techniques
Work EnvironmentLaboratories focused on genetic engineering and DNA assemblyResearch labs, biotech companies, or pharmaceutical settings
Industry UsageUsed in synthetic biology, genetic engineering, and researchInvolved in cloning, DNA manipulation, and genetic research
Job FocusDesigning and executing DNA assembly protocolsPreparing, executing, and verifying cloning procedures

Golden Gate Assembly and Cloning Technicians both work in molecular biology labs, often sharing similar educational backgrounds. However, Golden Gate Assembly specializes in a specific DNA assembly technique, while Cloning Technicians focus on cloning processes. Both roles are essential in genetic research and biotech industries, with overlapping skills but different primary responsibilities.

What are some common challenges faced when performing Golden Gate Assembly in a laboratory setting?

One of the main challenges in Golden Gate Assembly is designing compatible overhangs to ensure that DNA fragments ligate in the correct order, which requires careful planning and attention to detail. Another challenge is minimizing errors such as incomplete digestion or ligation, which can reduce the efficiency of the assembly process. Working with multiple fragments also increases the complexity, making troubleshooting and optimization essential skills. Collaboration with team members, such as molecular biologists or bioinformaticians, can help overcome these obstacles and improve overall success rates.
Research Scientist/Engineer 2, SeaHub

Research Scientist/Engineer 2, SeaHub

University of Washington

Seattle, WA • On-site

$90K/yr

Full-time

This job post has expired today. Applications are no longer accepted.

University Of Washington School Of Medicine rating

7.8

Company rating: 7.8 out of 10

Based on 9 frontline employees who took The Breakroom Quiz

174th of 532 rated colleges and universities

Job description

Job Description

The Department of Genome Sciences has an outstanding opportunity for a Research Scientist Engineer 2 to join the team.

About this Opportunity

Reporting to the Principal Investigator, the Research Scientist/Engineer 2, SeaHub, will perform molecular biology, cell biology, DNA sequencing, and data analysis experiments in the Pinglay lab. The Pinglay lab is focused on developing synthetic biology approaches to understand gene expression control, manipulate genome structure and engineer novel cellular states and is housed at the Seattle Hub for Synthetic Biology. ( https://www.pinglay-lab.com/ )

About Genome Sciences:

The Department of Genome Sciences (GS) is part of the University of Washington School of Medicine (UW SoM) and is comprised of 34 labs addressing leading edge questions in biology and medicine by developing and applying genetic, genomic, and computational approaches taking advantage of genomic information now available for a host of species. GS is making major contributions in the application of genome science to understand the fundamentals of biology and to improve human health and well-being.

Genome Sciences is a collaborative department supported by very different academic disciplines. We work with a multitude of Program Investigators (PIs) in departments both on the UW campus as well as worldwide. The department has gone through significant expansion since its beginning in 2001 with annual grant awards of $39M in FY25. The department has 63 training, teaching, and research faculty.

About the Seattle Hub for Synthetic Biology (SeaHub):

The SeaHub is a multi-institutional collaborative initiative between the Allen Institute, the Chan-Zuckerberg Initiative, and the University of Washington. The SeaHub is led by Scientific Director Dr. Jay Shendure and will work towards developing technologies for molecular recording in living cells. Beyond this technology mandate, the SeaHub will also serve as an organizing center for the burgeoning synthetic biology community in Seattle. SeaHub has cutting-edge scientific infrastructure including DNA sequencers, tissue culture spaces and laboratory automation.

Position Complexities

This researcher must be capable of developing and performing established molecular and cellular protocols and capable of performing tissue culture of mammalian cell lines in a Biosafety Level 2 (BSL2) environment, construct next-generation sequencing libraries, and basic data analysis. Strong organizational skills and a strong sense of initiative are also very important. There will be opportunities for publications as well as presentations at scientific meetings.

Dimensions and Impact to the University

This researcher will carry out work critical for the advancement of research projects in the Pinglay lab centered around using a combination of AI models and large-scale DNA synthesis to program desired gene expression patterns in mammalian systems. This researcher will also help further collaborative research projects both within and beyond UW.

Key Responsibilities

  • 30%: Develop and perform routine molecular and cellular protocols and biological tasks such as mammalian stem cell tissue culture in BSL2 environment and nucleic acid amplification.
  • 30%: Conducting an independent research project and/or furthering an existing research project in the lab with hands-on molecular and cellular biological work and DNA sequencing, including constructing next-generation sequencing libraries
  • 30%: Analyzing data and preparing presentation materials (e.g. figures, slides) and assisting with preparing publications and grant proposals. Present research at scientific meetings.
  • 10%: Assisting with lab organization, and upkeep.

Visa Eligibility

This position is eligible for H-1B sponsorship.

Requirements

Bachelor's degree in biology, molecular biology, genetics, or related field and two years of relevant experience, including working in a laboratory/research setting with experience in molecular biology workflows, cloning, and tissue culture processing in BSL2 environment, particularly stem cells.

Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Additional Requirements.

  • Experience in next generation sequencing library construction (RNAseq or ChIP-seq) with follow-up data analysis
  • Familiarity with DNA construction and assembly methods (PCR, Gibson, Golden Gate, ligation).
  • Bacterial transformation

Desired Qualifications

Experience with designing and implementing laboratory automation.

Experience with Python, R, or a similar programming language.

Demonstrated commitment to building an inclusive working and learning environment

Working Conditions

Wet BSL2 lab (70%), dry lab (30%)

Compensation, Benefits and Position Details

Pay Range Minimum:

$68,736.00 annual

Pay Range Maximum:

$90,000.00 annual

Other Compensation:

    Benefits:

    For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-uw-staff/

    Shift:

    First Shift (United States of America)

    Temporary or Regular?

    This is a regular position

    FTE (Full-Time Equivalent):

    100.00%

    Union/Bargaining Unit:

    UAW Research

    About the UW

    Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world.

    UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty.

    Our Commitment

    The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81 (https://policy.uw.edu/directory/po/executive-orders/eo-81-prohibiting-discrimination-harassment-and-sexual-misconduct/) .

    To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or dso@uw.edu .

    Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law (https://app.leg.wa.gov/RCW/default.aspx?cite=28B.112.080) .

    University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.

    Apply