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Golden Gate Assembly Jobs (NOW HIRING)

Uw

Research Scientist/Engr 1

Seattle, WA · On-site

$58K/yr

PCR, Gibson/Golden Gate assembly, mutagenesis, bacterial transformation, plasmid preparation, and sequence verification. Protein production: bacterial expression, affinity purification ...

Stowers Institute

Research Technician II

Kansas City, MO · On-site

$17.50 - $24/hr

Performing molecular cloning using techniques such as bacterial transformation, minipreps, PCR, gel electrophoresis, restriction digestion, Golden Gate assembly, and Gibson assembly. What We Offer

The Pennsylvania State University

$34K - $50K/yr

Training will be provided for techniques such as Golden Gate assembly and next-generation sequencing library preparation. * Manage bacterial cultures and perform growth assays. Prior experience with ...

Allen Institute

Research Associate I

Seattle, WA

$54K - $66K/yr

Experience performing molecular biology methods including molecular cloning (such as restriction cloning, Gibson Assembly, Golden Gate assembly, plasmid prep) * Experience cloning complex libraries ...

Allen Institute

Research Associate I

Seattle, WA · On-site

$54K - $66K/yr

Experience performing molecular biology methods including molecular cloning (such as restriction cloning, Gibson Assembly, Golden Gate assembly, plasmid prep) * Experience cloning complex libraries ...

Talent Software Services

Scientist I

San Diego, CA

Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT. Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter ...

Syensqo

Associate Scientist-Boston, MA

Boston, MA · On-site

$98K - $123K/yr

Design, build, and test recombinant DNA constructs , including plasmid construction, DNA assembly (Gibson Assembly, Golden Gate), cloning, transformation, and sequence verification. * Engineer ...

Gilead

Senior Scientist, Molecular Biology

Foster City, CA

$106K - $145K/yr

... assembly technologies including Gibson, Golden Gate, and In-Fusion cloning, with workflows tailored for scalable automation. * Lead the development and enhancement of high-throughput construct ...

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How much do golden gate assembly jobs pay per hour?

As of Jun 6, 2026, the average hourly pay for golden gate assembly in the United States is $16.91, according to ZipRecruiter salary data. Most workers in this role earn between $14.42 and $17.55 per hour, depending on experience, location, and employer.

What are the key skills and qualifications needed to thrive as a Molecular Biologist specializing in Golden Gate Assembly, and why are they important?

To excel as a Molecular Biologist working with Golden Gate Assembly, you need a solid background in molecular biology, genetics, and recombinant DNA techniques, often supported by a relevant degree such as biology or biochemistry. Familiarity with technical tools like PCR machines, DNA sequencing platforms, and specialized molecular cloning software is essential, along with experience in using Type IIS restriction enzymes. Strong attention to detail, problem-solving abilities, and effective collaboration skills distinguish top performers in this role. These skills ensure accurate genetic construct design, efficient workflow, and successful experimental outcomes in research and biotechnology applications.

What is Golden Gate Assembly?

Golden Gate Assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single reaction using type IIS restriction enzymes and DNA ligase. This technique is highly efficient and enables the precise and seamless assembly of genetic parts, such as promoters, coding sequences, and terminators, without leaving unwanted sequences at the junctions. It is widely used in synthetic biology and genetic engineering for building complex DNA constructs quickly and accurately.

What is the difference between Golden Gate Assembly vs Cloning Technician?

AspectGolden Gate AssemblyCloning Technician
CredentialsTypically requires molecular biology or biotechnology degreesRequires similar degrees or certifications in molecular biology or lab techniques
Work EnvironmentLaboratories focused on genetic engineering and DNA assemblyResearch labs, biotech companies, or pharmaceutical settings
Industry UsageUsed in synthetic biology, genetic engineering, and researchInvolved in cloning, DNA manipulation, and genetic research
Job FocusDesigning and executing DNA assembly protocolsPreparing, executing, and verifying cloning procedures

Golden Gate Assembly and Cloning Technicians both work in molecular biology labs, often sharing similar educational backgrounds. However, Golden Gate Assembly specializes in a specific DNA assembly technique, while Cloning Technicians focus on cloning processes. Both roles are essential in genetic research and biotech industries, with overlapping skills but different primary responsibilities.

What are some common challenges faced when performing Golden Gate Assembly in a laboratory setting?

One of the main challenges in Golden Gate Assembly is designing compatible overhangs to ensure that DNA fragments ligate in the correct order, which requires careful planning and attention to detail. Another challenge is minimizing errors such as incomplete digestion or ligation, which can reduce the efficiency of the assembly process. Working with multiple fragments also increases the complexity, making troubleshooting and optimization essential skills. Collaboration with team members, such as molecular biologists or bioinformaticians, can help overcome these obstacles and improve overall success rates.
Principal Research Associate/Associate Scientist, Molecular Assembly

Principal Research Associate/Associate Scientist, Molecular Assembly

TScan Therapeutics

Waltham, MA • On-site

Full-time

Posted 28 days ago

Job description

Position Summary:
TScan Therapeutics is a clinical-stage biotech seeking to revolutionize life-changing therapeutics for patients by identifying novel T cells and epitopes. We are looking for an experienced researcher to join our team as Principal Research Associate/Associate Scientist in the Molecular Assembly team, supporting our novel target identification and validation workflow in house. The ideal candidate should have relevant experience in cloning techniques and enjoy a fast-paced, collaborative, and vibrant culture. A team player with a sense of urgency will thrive in this role.
Responsibilities:
  • Collaborate with other scientists to design and build various constructs and vectors in support of target/TCR discovery efforts.
  • Assemble large libraries of peptides, TCRs, etc. using specialized cloning protocols.
  • Perform medium- to high-throughput cloning experiments to support discovery efforts in target identification and validation.
  • Analyze and interpret cloning and screening data (e.g., sequencing, functional assays) to guide construct design and optimization.
  • Troubleshoot complex cloning and library construction challenges and propose scalable solutions.
  • Lead group efforts towards technology development and improvement.
  • Implement and optimize high-throughput workflows, including automation where applicable.
  • Train co-workers on molecular cloning techniques.
  • Maintain accurate and well-documented experimental records (e.g., ELN) to ensure data integrity and reproducibility.

Requirements:
  • PhD with 0-2+ years of industry experience; Master's degree with 5-7+ years of relevant lab experience, preferably in the biopharmaceutical or biotechnology industry.
  • Hands-on experience in a variety of molecular cloning techniques, including Gibson Assembly, Golden Gate Assembly, Gateway recombination, etc.; experience constructing large-scale libraries is desirable.
  • Highly organized with very strong attention to detail.
  • Demonstrated ability to manage multiple projects and meet deadlines in a fast-paced environment.
  • Ability to work both independently and collaboratively and adapt quickly to changing priorities.
  • Scientific creativity, critical thinking, and problem-solving skills.
  • Excellent written and verbal communication skills, with the ability to document experiments in an organized and timely fashion and to present scientific data to coworkers.
  • Familiarity with sequence analysis tools, NGS, and basic bioinformatics workflows preferred.
  • Knowledge of immunology concepts, especially TCR biology and antigen recognition, is preferred.

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