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Enzyme Purification Jobs in Colorado (NOW HIRING)

University of Colorado

Post-Doctoral Fellow

Aurora, CO · On-site

$49K - $67K/yr

... in enzyme mechanisms. * Macromolecular assemblies involved in microbial metabolism with a ... Expertise in protein expression and purification How to Apply: For full consideration, please ...

University of Colorado

Post-Doctoral Fellow

Aurora, CO · On-site

$49K - $67K/yr

... in enzyme mechanisms. * Macromolecular assemblies involved in microbial metabolism with a ... Expertise in protein expression and purification How to Apply: For full consideration, please ...

All JobsEnzyme Purification JobsEnzyme Purification Jobs in Colorado

Enzyme Purification information

What is enzyme purification?

Enzyme purification is the process of isolating a specific enzyme from a complex mixture, such as a cell extract, to obtain it in a highly pure form. This typically involves a series of steps like centrifugation, filtration, precipitation, and chromatography to separate the enzyme from other proteins and contaminants. Purified enzymes are essential for research, industrial applications, and the development of diagnostic or therapeutic products.

What are the key skills and qualifications needed to thrive as an enzyme purification specialist?

To thrive as an Enzyme Purification Specialist, you need a solid background in biochemistry or molecular biology, experience with protein purification techniques, and often a relevant bachelor's or advanced degree. Familiarity with chromatography systems (such as HPLC or FPLC), spectrophotometers, and analytical software is typically required. Attention to detail, problem-solving abilities, and effective communication are soft skills that help ensure accuracy and collaboration. These skills are crucial for producing high-purity enzymes efficiently, ensuring reproducible results, and supporting research or manufacturing objectives.

What are some common challenges faced in enzyme purification roles, and how can they be addressed?

Professionals in enzyme purification often encounter challenges such as maintaining enzyme stability during extraction and purification, optimizing yield without compromising activity, and troubleshooting issues like protein aggregation or contamination. These challenges can be addressed by staying current with the latest techniques, regularly calibrating equipment, and collaborating closely with analytical and upstream processing teams. Effective communication and documentation are also crucial for resolving issues quickly and ensuring reproducibility in large-scale or GMP environments.

What is the difference between Enzyme Purification vs Protein Purification?

AspectEnzyme PurificationProtein Purification
CredentialsBiochemist, Molecular BiologistBiochemist, Molecular Biologist
Work EnvironmentLaboratories, biotech companiesLaboratories, biotech companies
Industry UsageEnzyme isolation for research and industrial useGeneral protein isolation for various applications
FocusIsolating and characterizing specific enzymesIsolating and analyzing diverse proteins

Enzyme Purification specifically involves isolating enzymes for research or industrial purposes, focusing on their activity and function. Protein Purification covers a broader range of proteins, including enzymes, structural proteins, and antibodies. While both roles require similar skills and work environments, enzyme purification emphasizes enzyme activity assays, whereas protein purification involves diverse techniques for different protein types.

What are popular job titles related to Enzyme Purification jobs in Colorado?

For Enzyme Purification jobs in Colorado, the most frequently searched job titles are:

What job categories do people searching Enzyme Purification jobs in Colorado look for?

The top searched job categories for Enzyme Purification jobs in Colorado are:

What cities in Colorado are hiring for Enzyme Purification jobs?

Cities in Colorado with the most Enzyme Purification job openings:

Enzyme Purification jobs near you

Postdoctoral Researcher - Enzyme engineering for cell-free systems

Golden, CO • On-site

National Renewable Energy Laboratory
Scientific Research and Development Services • 1 - 5K employees
Learn more about National Renewable Energy Laboratoryexternal

$76.60 - $126.40/hr

Other

Medical, Dental, Vision, Life, Retirement, PTO

Posted 7 days ago

Job description

Job Title

Postdoctoral Researcher – Enzyme Engineering for Cell‑Free Systems

Location

Golden, Colorado

Position Type

Postdoc (Fixed Term), 40 hours per week

Overview

RRES Center at NLR focuses on biological, chemical, and catalytic research in polymer design, upcycling, separations, synthetic biology, bioconversion, analytical sciences, computational modeling, and lignocellulosic chemistry. The successful candidate will work on the DOE‑funded project Integrating cell‑free systems and genome engineering to accelerate biosystems design for carbon‑negative biomanufacturing, developing optimized cell‑free systems for converting C1 substrates to sustainable biofuels and bioproducts.

Responsibilities
  • Identify, express, purify, and assay enzymes from full metabolic pathways.
  • Develop high‑throughput enzymatic assays to validate pathways.
  • Collaborate with data scientists to design efficient cell‑free production pathways.
  • Perform directed evolution, structure‑guided protein engineering, and machine‑learning‑guided protein engineering to improve enzyme and pathway function.
  • Conduct laboratory experiments safely and independently, with minimal supervision.
  • Plan and execute research in collaboration with multidisciplinary teams.
  • Communicate scientific results effectively in written and oral formats.
  • Author peer‑reviewed publications.
  • Work effectively in a large, multidisciplinary, multi‑institutional team of engineers and scientists.
Basic Qualifications
  • Recent PhD graduate within the last three years.
  • Experience with cloning, expression, and purification of proteins.
  • Experience with automation of biochemical assays.
  • Demonstrated experience in enzyme and/or protein engineering.
  • Ability to take initiative to set direction and goals for assigned tasks.
  • Strong troubleshooting and problem‑solving skills.
  • Strong record of quality peer‑reviewed publications.
  • Excellent safety record.
  • Ability to follow complex standard operating procedures with attention to detail.
Preferred Qualifications
  • Analytical chemistry experience with HPLC, GC‑FID, GC‑MS, and/or LC‑MS.
Annual Salary Range

$76,600 – $126,400

Benefits Summary
  • Medical, dental, and vision insurance.
  • Short‑term disability insurance.
  • Pension benefits.
  • 403(b) employee savings plan with employer match.
  • Life and accidental death and dismemberment insurance.
  • Personal time off and sick leave; paid holidays.
  • Possible performance‑, merit‑, and achievement‑based awards.
  • Possible relocation expense reimbursement.
Equal Opportunity Employer

All qualified applicants will receive consideration for employment without regard to age, color, disability, gender identity, genetic information, marital status, domestic partner status, military or veteran status, national origin, ancestry, race, religion, creed, sex, pregnancy, childbirth, breastfeeding, sexual orientation, or any other status protected by federal, state, or local law.

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