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Plasmid Vector Jobs (NOW HIRING)

... expression vectors, and platforms for protein and strain engineering. ATUM explores novel ... Purification of DNA plasmid * Restriction Digest of DNA * Work with automated liquid handlers

Design and test gene delivery approaches (AAV and/or other vectors, plasmid-based approaches, regulatory element/enhancer-directed expression as applicable). * Execute molecular biology workflows ...

Understanding of manufacturing processes for plasmid DNA and viral vectors in the cell & gene therapy industry. * Possess strong written and communication skills. * Proficient in Auto Cad & Microsoft ...

Additional duties include performing molecular biology and cell-based assays, plasmid cloning and preparation, and producing adeno-associated virus (AAV) vectors. The candidate may also support ...

... plasmid DNA construction and purification, protein purification and measurements of virus ... Construct and purify new cloned DNAs for subsequent incorporation into recombinant AAV vectors.

... plasmid DNA construction and purification, protein purification and measurements of virus ... Construct and purify new cloned DNAs for subsequent incorporation into recombinant AAV vectors.

Quality Assurance Associate III

Bothell, WA ยท On-site

$74K - $103K/yr

S., and Japan, we deliver development and manufacturing of mammalian and microbial-based therapeutic proteins, plasmid DNA (pDNA), messenger RNA (mRNA), viral vectors, and genetically engineered ...

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Plasmid Vector information

Is Addgene reliable?

Addgene is a reputable nonprofit organization that supplies plasmid vectors to researchers worldwide. It is widely trusted in the scientific community for its quality control, transparent sharing policies, and accurate plasmid documentation. Researchers often rely on Addgene for obtaining verified plasmids for their experiments.

What are some common challenges faced when working as a Plasmid Vector Scientist in a research laboratory?

As a Plasmid Vector Scientist, one of the most common challenges is ensuring the accuracy and efficiency of cloning and vector construction, which often involves troubleshooting issues like low yield, contamination, or unexpected mutations. Managing multiple projects simultaneously and keeping up with rapidly evolving molecular biology techniques can also be demanding. Collaboration with other scientists, such as molecular biologists and bioinformaticians, is essential to streamline workflows and achieve research goals. Staying organized, maintaining meticulous records, and being adaptable to new protocols are key to overcoming these challenges.

What jobs can I do with molecular biology?

With a background in molecular biology, you can pursue roles such as research scientist, laboratory technician, or molecular biologist, often working with techniques like DNA cloning, PCR, and plasmid vector manipulation. These jobs typically require laboratory skills, knowledge of genetic techniques, and sometimes relevant certifications or advanced degrees.

What is the difference between Plasmid Vector vs Cloning Technician?

AspectPlasmid VectorCloning Technician
Required CredentialsBachelor's in Molecular Biology or related field, lab experienceAssociate's or Bachelor's, hands-on lab skills in cloning
Work EnvironmentResearch labs, biotech companiesResearch labs, biotech manufacturing
Industry UsageGenetic engineering, molecular cloningCloning, DNA manipulation, sample preparation

While a plasmid vector is a DNA molecule used to transfer genetic material, a cloning technician performs laboratory tasks involving DNA cloning techniques. The plasmid vector is a tool, whereas the cloning technician is a professional who uses such tools in laboratory settings to achieve genetic research or product development.

What are plasmid vectors?

Plasmid vectors are small, circular DNA molecules that are used in molecular biology to transfer genetic material into a host cell. They are engineered to contain features such as an origin of replication, selectable markers, and multiple cloning sites, which make them useful tools for cloning, gene expression, and genetic engineering experiments. Plasmid vectors are commonly used to produce proteins, study gene function, and develop genetically modified organisms. Because they can replicate independently within host cells, they are an essential tool in biotechnology and genetic research.

What are the key skills and qualifications needed to thrive as a Plasmid Vector, and why are they important?

I'm sorry, but 'Plasmid Vector' is not a real-world professional occupation and therefore cannot be described in terms of job skills and qualifications.

What is the job of a plasmid?

A plasmid is a small, circular DNA molecule used in genetic engineering to carry and replicate specific genes within a host organism. The role of a plasmid in a laboratory setting involves cloning, gene expression, and genetic modification, often requiring skills in molecular biology techniques and the use of laboratory tools. Understanding plasmid functions is essential for jobs in biotechnology and research environments.

What do plasmid vectors do?

Plasmid vectors are DNA molecules used in molecular biology to carry genetic material into host cells, typically bacteria, for cloning, gene expression, or genetic modification. They enable scientists to replicate, manipulate, and study specific genes efficiently in laboratory settings.
Infographic showing various Plasmid Vector job openings in the United States as of June 2026, with employment types broken down into 100% Full Time. Highlights an 66% Physical, 4% Hybrid, and 30% Remote job distribution.
Scientist II, Cell Line Engineering

Scientist II, Cell Line Engineering

BigHat Biosciences

San Mateo, CA โ€ข On-site

$145K - $165K/yr

Full-time

Medical, Dental, Vision, Retirement

Posted 19 days ago


Job description

Scientist II, Cell Line Engineering
Department: Lab Science
Employment Type: Full Time
Location: San Mateo, CA
Description
The role: BigHat is looking for an energetic and motivated individual to lead cell line engineering as a core Platform capability in support of our pipeline Discovery and early R&D efforts. This is a lab based role that will report directly to the Head of Protein Sciences, work cross functionally with Programs and Discovery, and directly impact the identification and validation of next generation biotherapeutics engineered on the Milliner Platform.
*At BigHat we believe in titles that commensurate with skill set, relative organizational impact, and value contribution; more experienced candidates are encouraged to apply, with the understanding that responsibilities and title would adjust as appropriate.
Key Responsibilities
  • Onboard all the core components necessary for engineering cell lines for expressing pipeline targets including expression vectors, gene delivery methods, selective antibiotic concentration determination protocols, expression validation and cell sorting capabilities
  • Culture and maintain a variety of cell types; generate diverse target cell lines including knock-outs, stable cell lines overexpressing one or more targets, and reporter cell lines
  • Perform the validation of cell line expression using flow and sequencing (Sanger or NGS) based methods.
  • Generate bulk pool and single cell populations utilizing FACS to select for cell lines of various expression levels
  • Maintain an organized cell line inventory with all relevant documentation
  • Investigate target expression biology to recapitulate the most native conditions for expression.
  • Work with end user stakeholders to confirm correct domain expression and associated sequences
  • In collaboration with end user stakeholders, establish the workflows, timelines and deliverables for cell line engineering from request to hand-off
  • Collaborate with automation engineers to automate routine cell culture workflows to increase efficiencies and throughput

Skills Knowledge and Expertise
  • PhD in biology-related discipline with minimum of 3+ years industry experience, preferably in an environment utilizing cell lines for antibody discovery and engineering
  • In depth molecular biology knowledge for plasmid construction and experience selecting expression vectors and promoter types
  • Experience culturing various cell types, including adherent, suspension, shaker flask etc. High level of organization in handling multiple cell lines simultaneously for manipulation and maintenance
  • Demonstrated hands-on success in using CRISPR/Cas9, transposon, or lentivirus based technologies
  • Adept with Fluorescence or magnetic-bead based cell sorting methods, and clonal selection and maintenance
  • Prior use of automation for cell culture a plus

Total Rewards
The salary estimated for this position is $145,000 - $165,000 + bonus + options + benefits. Compensation will vary depending on job-related knowledge, skills, and experience. Actual compensation will be confirmed in writing at the time of the offer.
What BigHat Offers:
  • Range of health insurance plan options through Anthem and Kaiser (monthly credit if benefit waived)
  • Dental, and vision coverage through Guardian
  • Additional well-being benefits through Nayya, OneMedical, Wagmo, Rula, and more
  • 401(k) with company match
  • DTO, two weeks of company-wide shutdown, and 12 company holidays
  • Paid parental leave