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In Situ Hybridization Jobs in Indiana (NOW HIRING)

Indiana University

Postdoctoral Fellow

Bloomington, IN · On-site

$45K - $61K/yr

Experience with cell culture, immunofluorescence or in situ hybridization would be considered a plus. Department Contact for Questions Questions related to this job posting can be directed to Maria ...

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In Situ Hybridization information

What is the difference between In Situ Hybridization vs Cytogenetic Technologist?

AspectIn Situ HybridizationCytogenetic Technologist
Required CredentialsTypically requires a bachelor's degree in biology, molecular biology, or related field; certification may be preferredRequires a bachelor's degree in biology, genetics, or related field; certification often recommended
Work EnvironmentLaboratory settings focusing on molecular and cellular analysisLaboratories performing chromosome analysis and genetic testing
Industry UsageUsed in research, diagnostics, and pathology labs for detecting nucleic acid sequencesUsed in clinical genetics labs for karyotyping and genetic diagnosis

In Situ Hybridization and Cytogenetic Technologists both work in laboratory settings within the genetics and molecular biology fields. While In Situ Hybridization focuses on detecting specific nucleic acid sequences within cells, Cytogenetic Technologists analyze chromosomes for genetic abnormalities. Both roles require similar educational backgrounds and are essential in genetic diagnostics, but they specialize in different techniques and analysis methods.

What are the key skills and qualifications needed to thrive as an in situ hybridization specialist?

To thrive as an In Situ Hybridization Specialist, you need a solid background in molecular biology, histology, and laboratory techniques, usually supported by a degree in a biological science. Proficiency in using laboratory instruments such as microtomes, microscopes, and imaging systems, as well as familiarity with protocols like FISH and RNAscope, is essential. Attention to detail, problem-solving skills, and effective communication are valuable soft skills that enhance accuracy and collaboration. These qualifications ensure precise experimental results, reliable data interpretation, and successful teamwork in research or diagnostic settings.

What are some common challenges faced by professionals working in in situ hybridization, and how can they be addressed?

Professionals working in In Situ Hybridization often encounter challenges such as optimizing probe specificity, minimizing background staining, and maintaining sample integrity. These issues can affect the clarity and reliability of results. Addressing them typically involves rigorous protocol optimization, careful reagent selection, and thorough troubleshooting. Additionally, collaboration with pathologists, molecular biologists, and lab technicians is crucial for interpreting results and refining techniques. Continuous training and staying updated on new ISH methodologies can further help overcome these challenges.

What is in situ hybridization?

In situ hybridization (ISH) is a laboratory technique used to detect specific nucleic acid sequences (DNA or RNA) within fixed tissues or cells. This method uses labeled probes that bind to complementary sequences, allowing researchers to visualize the location, abundance, and distribution of target genes or transcripts directly in their cellular context. ISH is widely used in research and diagnostics to study gene expression patterns, identify genetic abnormalities, and investigate infectious agents in tissue samples.

How to do in situ hybridization?

In situ hybridization is a laboratory technique used by molecular biologists to detect specific nucleic acid sequences within tissue sections or cell samples. The process involves preparing samples, applying a labeled complementary DNA or RNA probe, hybridizing under controlled conditions, and then visualizing the bound probe using microscopy. Proper technique, probe design, and adherence to safety protocols are essential for accurate results.
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In Situ Hybridization jobs near you
Infographic showing various In Situ Hybridization job openings in Indiana as of August 2026, with employment types broken down into 96% Full Time, 3% Part Time, and 1% Contract. Highlights an 94% Physical, 3% Hybrid, and 3% Remote job distribution.

Postdoctoral Fellow

Indiana University

Bloomington, IN • On-site

$45K - $61K/yr

Full-time

Re-posted 20 days ago

Job description

Posting Details
Position Details
Title
Postdoctoral Fellow
Specific Title
Appointment Type
Postdoctoral Fellow
Department
IUSM - Medical & Molecular Genetics
Campus
IU School of Medicine Indianapolis
Position Summary
The Department of Medical and Molecular Genetics at Indiana University School of Medicine is seeking applicants at the Postdoctoral Fellow level. The Ware lab investigates the genetic and developmental basis of pediatric heart disease. We analyze genetic variation in samples from patients with congenital heart defects and utilize mouse, Xenopus and cell-based models to assess these variants. Our lab also uses these models to study genes previously linked to congenital cardiac defects, with a particular focus on left-right patterning genes.
IU School of Medicine is committed to being a welcoming campus community and we seek candidates whose research, teaching, and community engagement efforts contribute to robust learning and working environments for all students, staff, and faculty. We invite individuals who will join us in our mission to improve health equity and well-being for all throughout the state of Indiana.
Applications will be received until the position is filled.
Stephanie Ware, MD, Phd (https://medicine.iu.edu/faculty/22816/ware-stephanie) is the Chair of the Department of Medical and Molecular Genetics as well as the program leader of the Cardiovascular Genetics Program within the Wells Center of Pediatric Research. Her long-term research goal is to identify causes of congenital heart defects.
Basic Qualifications
The successful candidates must have a Ph.D. degree with a strong research background in genetics and/or developmental biology. Candidates should have experience with mouse or Xenopus models and molecular biology techniques. Experience collecting/processing mouse embryos, particularly at early stages of development, is highly desirable. Experience with cell culture, immunofluorescence or in situ hybridization would be considered a plus.
Department Contact for Questions
Questions related to this job posting can be directed to Maria Padua: mpadua@iu.edu
Additional Qualifications
The candidates must be self-motivated, well-organized and comfortable mentoring graduate students in basic techniques. A successful candidate must have experience collecting/analyzing/interpreting data and will be expected to participate in manuscript preparation and grant development. It is highly desirable for the holders of this position to develop research projects with strong potential for long-term NIH funding, and to have demonstrated the ability to collaborate with others.
Example publications • Congenital heart defects caused by FOXJ1 https://pubmed.ncbi.nlm.nih.gov/37158461/
• Loss of Zic3 impairs planar cell polarity leading to abnormal left-right signaling, heart defects and neural tube defects https://pubmed.ncbi.nlm.nih.gov/34274973/
Special Instructions
Priority Application Review Deadline
Expected Start Date
Posting Number
IUSM-02353-2026

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