Full Time Transgenic Mouse Jobs in Florida (NOW HIRING)

• Independently design, perform, and troubleshoot experiments investigating mechanisms of pulmonary inflammation, immune regulation, tissue repair, and resolution biology.

• Perform murine models of lung injury and disease, including bacterial pneumonia, acute lung injury, ARDS, asthma, lung cancer, environmental exposure models, and sleep-related inflammatory models.

• Conduct animal procedures including intratracheal instillation, intranasal administration, bronchoalveolar lavage, tissue harvesting, blood collection, and survival studies.

• Isolate and characterize immune cell populations from lung, lymphoid tissues, blood, and bronchoalveolar lavage specimens.

• Design, optimize, and execute high-parameter spectral flow cytometry panels (25-40 colors) for comprehensive immune phenotyping.

• Perform cell sorting and downstream molecular analyses of macrophages, T regulatory cells, monocytes, neutrophils, dendritic cells, and other immune populations.

• Execute molecular biology techniques including RNA isolation, qPCR, bulk RNA sequencing sample preparation, single-cell RNA sequencing workflows, TCR sequencing, and gene expression analyses.

• Conduct cell culture experiments involving primary macrophages, T lymphocytes, epithelial cells, endothelial cells, and immortalized cell lines.

• Perform advanced immunologic assays including phagocytosis, cytokine profiling, proliferation assays, intracellular signaling studies, and functional immune cell characterization.

• Assist with spatial transcriptomics, confocal microscopy, immunofluorescence imaging, and image analysis.

• Maintain breeding colonies and perform genotyping of transgenic and conditional knockout mouse models.

• Analyze experimental data using FlowJo, Prism, R, and other bioinformatic platforms.

• Maintain detailed laboratory records, experimental databases, standard operating procedures, and quality-control documentation.

• Prepare figures, datasets, protocols, abstracts, manuscripts, and grant-related materials for publication and presentation.

• Train and supervise students, technicians, fellows, and research staff in laboratory techniques and animal procedures.

• Coordinate laboratory operations including inventory management, equipment maintenance, biosafety compliance, IACUC protocols, and reagent procurement.

• Collaborate with investigators, core facilities, and external research partners to support multidisciplinary translational research projects.

This list of duties and responsibilities is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary.