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This Research Assistant position is available in the laboratory of Dr. Mary Weber in the Department of Microbiology and Immunology. The successful candidate will contribute to cutting-edge research focused on understanding how the intracellular bacterial pathogens Chlamydia trachomatis and Orientia tsutsugamushi use secreted effector proteins to manipulate host cell processes and promote infection.

Research projects will utilize a multidisciplinary approach incorporating molecular biology, cell biology, biochemistry, microbiology, and genetic techniques.

Position Responsibilities: 

• Perform library and online research on specified topics for use in experimentation.

• Perform experimental studies based on given hypotheses and goals; evaluate and record outcomes of experiments based on hypotheses and goals and submit for investigator's inspection.

• Assist in the analysis and interpretation of experiments.

• Observe techniques in other laboratories for implementation in our laboratory.

• Complete work within specified time guidelines; discuss progress and any problems as needed.

• Maintain and passage multiple cell lines.

• Purify chlamydia from infected cells by density gradient.

• Use immunofluorescent microscopy to titer bacterial stocks.

• Perform molecular biology experiments including cloning, construction of vectors for expression in chlamydial and make mutants using site-specific gene inactivation.

• Prepare chemical solutions and media according to established protocols.

• Maintain bacterial and eukaryotic stocks and inventory.

• Present research progress at laboratory and journal club meetings.

• Assist in the preparation of manuscripts and grants by organizing data and preparing images, figures, and tables as directed.

• Assist in completion of required paperwork for new and departing laboratory personnel, including annual training, biohazard safety, laboratory safety, and fire safety training.

• Assist in maintaining accurate records of laboratory databases (equipment and supplies, reagents, microbial strains and stocks, and animals).

• Maintain laboratory supplies and equipment, including the timely ordering, receiving, and proper storage of supplies and equipment.

• Assist in maintaining general laboratory functionality.

• Assist in developing new protocols.

• Maintain a clean work area.

• May assist in the BSL3

• May assist in animal facilities

Percent of Time: 100%

Staff Type: Professional & Scientific

Type of Position: Specified Term. Initial appointment is for one year. Appointment may be extended based on performance and availability of funding.

Pay Grade: 3A -  https://hr.uiowa.edu/professional-pay/compensation-plan-structure-a-FY18

Benefits Highlights:

Regular salaried position located in Iowa City, Iowa

• Fringe benefit package including paid vacation; sick leave; health, dental, life and disability insurance options; and generous employer contributions into retirement plans

• For more information about Why Iowa?, click here

Required Qualifications:

• Requires a bachelor's degree in Microbiology, Molecular Biology, Cell Biology, or Immunology, or a related field or equivalent experience

• Excellent written and verbal communication skills

• Basic knowledge of each of the following:

  • General microbiologic techniques, such as culture, storage, and safe handling of pathogenic agents.

  • Standard molecular techniques, including PCR, plasmid purification, bacterial transformation, agarose gel electrophoresis, and western blotting.

  • Gathering, recording, and analyzing of experimental data.

  • Data analysis and presentation software, such as Microsoft Excel, Prism, Microsoft Word, and Photoshop

• Proficiency with each of the following:
  • Ability to effectively prioritize projects to consistently meet deadlines
  • Ability to multitask in a fast-paced environment 
  • Attention to detail and accuracy
  • Ability to work in a team

Desirable Qualifications:

• Prior experience working with pathogenic microorganisms is desired
• Prior experience with each of the following:
  • Tissue culture experience
  • Immunofluorescence microscopy
  • Western Blotting
  • Cloning

Application Process: In order to be considered, applicants must upload a CV or resume, and cover letter (under submission relevant materials) that clearly address how they meet the listed required and desired qualifications of this position.

Job openings are posted for a minimum of 7 calendar days and may be removed from posting and filled any time after the original posting period has ended. Successful candidates will be required to self-disclose any conviction history and will be subject to a criminal background check and credential/education verification. This position is not eligible for University sponsorship for employment authorization. Professional references will be requested at a later step in the recruitment process.

For additional questions, please contact Anne Beyerink at anne-phillips@uiowa.edu.