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Discover Vanderbilt University Medical Center: Located in Nashville, Tennessee, and operating at a global crossroads of teaching, discovery, and patient care, VUMC is a community ofindividuals who come to work each day with the simple aim of changing the world. It is a place where your expertise will be valued, your knowledge expanded, and your abilities challenged. Vanderbilt Health is committed to an environment where everyone has the chance to thrive and where your uniquenessis sought and celebrated. It is a place where employees know they are part of something that is bigger than themselves, take exceptional pride in their work and never settle for what was good enough yesterday. Vanderbilt's mission is to advance health and wellness through preeminent programs in patient care, education, and research.

Organization:

PMI Molecular Pathogenesis

Job Summary:

Our laboratory in the Department of Pathology, Microbiology & Immunology seeks a curious, motivated and diligent Research Assistant I or II to work on patient-based immunology projects. The Markle lab seeks to understand genetic drivers of immunological diseases by identifying rare gene variants in affected children and studying how they impact the immune system. This is a research lab-based position that will involve both performing experimental work at the bench and also supporting other lab members with their projects.
Required:
- BSc in microbiology/immunology, biochemistry, genetics, cell biology or similar area
- organized, reliable, attention to detail
- strong communication skills
- willingness to learn a variety of techniques at the bench
Not required, but ideal candidates will:
- have experience with some of the following techniques: DNA extraction, PCR, cloning, sterile cell culture, flow cytometry, western blotting, ELISA, fluorescent or confocal microscopy.
- commit to a minimum of 2 years of full-time employment. Those who wish to pursue medical school or a PhD in the long term are welcome.
We offer a dynamic and collaborative research environment, have opportunities for authorship and presentation of your work, and support diversity in science. You will receive lots of mentorship and will gain skills applicable across many fields of science and medicine. Recent graduates with some relevant lab experience are encouraged to apply.
This position will work on site at Vanderbilt Medical Center North located on 21st Ave. in Nashville, TN and will work Monday-Friday, standard daytime business hours.

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DEPARTMENT SUMMARY:

The Markle Lab is interested in understanding genetic determinants of immunity. Our studies are patient-based, and we focus on children with severe immune-mediated diseases. Our lab uses human whole exome and whole genome sequencing to discover monogenic (e.g. single gene) inborn errors of immunity in children with rare infectious, autoimmune, or auto-inflammatory diseases. We use a customized bioinformatic pipeline to sift through large genetic datasets to pinpoint candidate gene variants. Then, we study the impact of these variants on the expression and function of the encoded proteins. We aim to thoroughly characterize the functional impact of each variant at the molecular and cellular levels, and to this end we use both classical and cutting-edge techniques in molecular biology, protein biochemistry, in vitro cell culture approaches, and mass cytometry using patient leukocytes. Current projects include: inflammasome-activating mutations, mutations affecting cytokine receptors, novel candidate genes for intestinal inflammation, inflammatory skin diseases, and characterizing the microbiota of patients with monogenic immune diseases. Our group uses both 'dry-lab' (computational) and wide variety of 'wet-lab' approaches and our interests lie at the crossroads of human genetics, human immunology, and host-microbe interactions.

KEY RESPONSIBILITIES
Under supervision, run experiments, assays, and record results
Read and follow experimental protocols (May include animal handling, tissue culture, etc.)
Compile and communicate results to supervisor
Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogenics)
Perform calculations involving molarity, % solutions, and serial dilutions
Prepare and operate equipment in experimental procedures
May include blood gas analyzer, centrifuge, pH meter, spectrophotometer, autoclave, scintillation counter, electrophoresis unit, analytical balance, etc.
Maintain and repair equipment according to manufacturer and university guidelines.
Ensure Laboratory is prepared for experiments
Maintain inventories,
Maintain sterile conditions as required
Prepare reagents
Clean equipment.
TECHNICAL CAPABILITIES

Our Academic Enterprise is one of the nation's longest serving and most prestigious academic medical centers. Through its historic bond with Vanderbilt University and integral role in the School of Medicine, Vanderbilt Health cultivates distinguished research and educational programs to advance a clinical enterprise that provides compassionate and personalized care and support for millions of patients and family members each year.

World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.

Aligning with Vanderbilt Health'sStrategic Directions, the Office of Research providesshared research resources,administrative expertiseand professional guidance to enablethetrans-disciplinary environment and highly collaborative culture that advances discovery and training forall the research faculty, trainees, students and staff.

Core Accountabilities:

Organizational Impact: Delivers job responsibilities that impact own job area/team with some guidance. Problem Solving/ Complexity of work: Uses existing procedures, research and analysis to solve standard job related problems that may require some judgement. Breadth of Knowledge: Requires subject matter knowledge within a professional area to meet job requirements. Team Interaction: Individually contributes to project/ work teams.

Core Capabilities :

Supporting Colleagues: - Develops Self and Others: Continuously improves own skills by identifying development opportunities.- Builds and Maintains Relationships: Seeks to understand colleagues priorities, working styles and develops relationships across areas. - Communicates Effectively: Openly shares information with others and communicates in a clear and courteous manner. Delivering Excellent Services: - Serves Others with Compassion: Invests time to understand the problems, needs of others and how to provide excellent service. - Solves Complex Problems: Seeks to understand issues, solves routine problems, and raises proper concerns to supervisors in a timely manner. - Offers Meaningful Advice and Support: Listens carefully to understand the issues and provides accurate information and support. Ensuring High Quality: - Performs Excellent Work: Checks work quality before delivery and asks relevant questions to meet quality standards.- Ensures Continuous Improvement: Shows eagerness to learn new knowledge, technologies, tools or systems and displays willingness to go above and beyond. - Fulfills Safety and Regulatory Requirements: Demonstrates basic knowledge of conditions that affect safety and reports unsafe conditions to the appropriate person or department. Managing Resources Effectively :- Demonstrates Accountability: Takes responsibility for completing assigned activities and thinks beyond standard approaches to provide high-quality work/service. - Stewards Organizational Resources: Displays understanding of how personal actions will impact departmental resources. - Makes Data Driven Decisions: Uses accurate information and good decision making to consistently achieve results on time and without error. Fostering Innovation: - Generates New Ideas: Willingly proposes/accepts ideas or initiatives that will impact day-to-day operations by offering suggestions to enhance them.- Applies Technology: Absorbs new technology quickly; understands when to utilize the appropriate tools and procedures to ensure proper course of action. - Adapts to Change: Embraces changes by keeping an open mind to changing plans and incorporates change instructions into own area of work.

Position Qualifications:

Responsibilities:

Certifications :

Work Experience :

Relevant Work Experience

Experience Level :

Less than 1 year

Education :

Bachelor's

This role offers the opportunity to make a meaningful impact within Vanderbilt Health, supported by a comprehensive benefits package which mayinclude health, disability, retirement and/or wellness offerings to enhance your well-being and professional growth.

Vanderbilt Health is committed tofosteringan environment where everyone has the chance to thrive and is committed to the principles of equal opportunity. EOE/Vets/Disabled.