Single Cell Spatial Transcriptomics Jobs in Missouri

Thanks for your interest in Children's Mercy!
Do you envision finding a meaningful role with an inclusive and compassionate team? At Children's Mercy, we believe in making a difference in the lives of all children and shining a light of hope to the patients and families we serve. Our employees make the difference, which is why we have been recognized by U.S. News & World Report as a top pediatric hospital, for eleven consecutive years.
Children's Mercy is in the heart of Kansas City - a metro abounding in cultural experiences, vibrant communities and thriving businesses. This is where our patients and families live, work and play. This is a community that has embraced our hospital and we strive to say thanks by giving back. As a leader in children's health, we engage in meaningful programs and partnerships throughout the region so that we can improve the lives of children beyond the walls of our hospital.
Overview
The Neonatal Diseases Research Program (Director: Dr. Venkatesh Sampath) and the Division of Neonatology (Director: Dr. Steve Olsen) are looking to hire a PhD scientist to conduct translational/ basic science research in the areas of neonatal lung injury and lung developmental biology. The NDRP, which focuses broadly on the molecular mechanisms regulating endothelial and epithelial injury in the context of the developing lung and intestine, is funded by the NHLBI, NICHD, NIDDK, research foundations, and institutional funds. The laboratory uses transgenic mouse models, human genetics, cell-culture models, microbiome analyses, and novel therapeutics to study the inflammatory/ developmental basis of diseases such as bronchopulmonary dysplasia, necrotizing enterocolitis, and infectious diseases. The laboratory currently employs 5 full time PhD and masters level scientists. Translational research endeavors are supported by neonatal research coordinators. Faculty and clinical fellows from Neonatology as well as faculty from infectious disease and immunology participate in the NDRP. We advocate a culture of discovery, innovation, team science and excellence. Access to an active neonatal genomic biorepository established by Dr. Sampath, that houses de-identified DNA samples from phenotypically well-characterized preterm cohorts, is available. The Neonatal Diseases Research Program (NDRP) laboratories has >1000 sq. feet of wet lab space located on the west side of 7th floor of Children's Mercy Research Institute, a modern state-of-art research tower that houses 375,000 total sq. ft of research space. Apart from individual labs, the CMRI houses research cores that support genomics, flow cytometry, imaging, GMP biologics, and is developing a rodent vivarium.
We are looking for a highly motivated individual with advanced molecular and cell biology techniques to study endothelial/epithelial injury in the context of the developing lung. Specific expertise in endothelial/vascular biology, Crispr/Cas9 gene targeting, use of single cell RNA-sequencing and bioinformatics and rodent work in the lung is preferred. Minimal requirements include a PhD degree with 2-5 years of post-graduate experience. The qualified applicant will work as part of a vibrant team consisting of physician-scientists, research technicians and PhD scientists under the guidance of Dr. Sampath. This position is available for an initial period of 2 years.
At Children's Mercy, we are committed to ensuring that everyone feels welcomed within our walls. A successful candidate for this position will join us as we strive to create a workplace that reflects the community we serve, as well as our core values of kindness, curiosity, inclusion, team and integrity.
Additionally, it's important to us that we remain transparent with all potential job candidates. Because we value the safety of the patients and families we serve, as well as the Children's Mercy staff, we want to let you know that the seasonal influenza vaccine is a condition of employment for all employees in our organization. New employees must be willing to be vaccinated if found non-immune to measles, mumps, rubella (MMR) and chicken pox (varicella) and/or without evidence of tetanus, diphtheria, acellular pertussis (Tdap) vaccination since 2005. If you are selected for this position, you will be asked to supply your immunization records as proof of vaccination. If you and have any concerns about receiving these vaccines, medical and/or religious exemptions can be further discussed with Human Resources.
Responsibilities

• Writes and executes protocols for experiments. Organizes, analyzes, and summarizes acquired data, using scientific and statistical techniques, manuscript writing (required)
• Single cell RNA sequencing, transcriptomics, and computational analysis tools (preferred).
• Molecular biology - Cloning, nucleic acid purifications, Sequencing, CoIP, ChIP, Southern and Northern blot, Western blot, In vitro phosphatase assay, Protein purification, ELISA (preferred).
• Cell biology - Stem cell isolation and propagation, cell line creation, cell phenotyping by FACS analysis, cell culture and endothelial cell culture, drug treatment, Cell Transfection (preferred)
• Animal models - Making transgenic constructs (knock-out/in and conditional knock-out), Mouse embryo dissection and organ mirco-dissection (preferred)
• Experience in working in mice (genotyping, drug treatment etc) is required.
• Expertise in endothelial/vascular signaling and lung biology (preferred)
• Ability to work with trainees and guide them through their projects
• Writes and executes protocols for experiments. Organizes, analyzes, and summarizes acquired data, using scientific and statistical techniques, manuscript writing (required)
• Single cell RNA sequencing, transcriptomics, and computational analysis tools (preferred).
• Molecular biology - Cloning, nucleic acid purifications, Sequencing, CoIP, ChIP, Southern and Northern blot, Western blot, In vitro phosphatase assay, Protein purification, ELISA (preferred).
• Cell biology - Stem cell isolation and propagation, cell line creation, cell phenotyping by FACS analysis, cell culture and endothelial cell culture, drug treatment, Cell Transfection (preferred)
• Animal models - Making transgenic constructs (knock-out/in and conditional knock-out), Mouse embryo dissection and organ mirco-dissection (preferred)
• Experience in working in mice (genotyping, drug treatment etc) is required.
• Expertise in endothelial/vascular signaling and lung biology (preferred)
• Ability to work with trainees and guide them through their projects

Qualifications

• Ph.D. or MD or related doctoral level degree in a relevant discipline and 1-2 years experience

Benefits at Children's Mercy
The benefits plans at Children's Mercy are one of many reasons we are recognized as one of the best places to work in Kansas City. Our plans are designed to meet the changing needs of our employees and their families.
Learn more about Children's Mercy benefits.
Starting Pay
Our pay ranges are market competitive and following the NIH guidelines.
Remote Work/Work from Home
This position is not eligible to work remotely, which means that the person hired will be required to work onsite at one of our Children's Mercy locations and may not work from home.
EEO Employer/Disabled/Vet
Children's Mercy hires individuals based on their job skills, expertise and ability to maintain professional relationships with fellow employees, patients, parents and visitors. A personal interview, formal education and training, previous work experience, references and a criminal background investigation are all factors used to select the best candidates. The hospital does not discriminate against prospective or current employees based on the race, color, religion, sex, national origin, age, disability, creed, genetic information, sexual orientation, gender identity or expression, ancestry or veteran status. A drug screen will be performed upon hire. Children's Mercy is smoke and tobacco free.
CM is committed to creating a workforce that supports the diverse backgrounds of our patients and families. We know that our greatest strengths come from the people who make up our team, so we hire great people from a wide variety of backgrounds, not just because it's the right thing to do, but because it makes our hospital stronger and our patient care more compassionate.
If you share our values and our enthusiasm for service, you will find a home at CM. In recruiting for our team, we welcome the unique contributions that you can bring, including education, ideas, culture, and beliefs.