The Advanced Scientist / Senior Sequencing Specialist plays a key role in the USF Genomics Sequencing Core, supporting Illumina Next-Generation Sequencing (NGS) and advanced single-cell and spatial transcriptomics sample preparation. This full-time position operates on a 9:00 AM-5:00 PM schedule and is responsible for ensuring efficient, high-quality daily operations within the Core. The incumbent will perform hands-on sequencing workflows, maintain rigorous quality standards, and ensure timely delivery of sequencing results while implementing best practices for reproducible and high-quality data generation.
Responsibilities
Responsibilities:
- Perform Next-Generation Sequencing (NGS) sample preparation and sequencing, including single-cell RNA-seq (scRNA-seq, snRNA-seq) and spatial transcriptomics (Visium HD) workflows, DNA/RNA library construction, Illumina sequencing, Nanopore sequencing, and Visium HD tissue sample preparation.
- Operate and maintain high-throughput sequencing instruments (e.g., Illumina NovaSeq X, MiSeq i100) and single-cell systems (e.g., 10x Genomics Chromium X, Visium CytAssist).
- Manage and prioritize daily laboratory workflow to ensure timely project completion within standard operating hours.
- Conduct quality control (QC) assessments for RNA/DNA samples and sequencing libraries to ensure accuracy and integrity. Work closely with core users and internal team to troubleshoot, optimize protocols, and achieve consistent, high-quality results.
- Maintain accurate documentation in iLab, including experimental records, billing, reagent usage, and equipment logs, ensuring compliance with institutional standards.
- Contribute to laboratory training, SOP development, and continuous process improvement initiatives to enhance efficiency and performance.
Qualifications
Minimum Qualifications:
- Master's degree in Molecular Biology, Genomics, or a closely related field, and at least two (2) years of professional experience in a research or technical laboratory;
or
- Bachelor's degree in a related field and at least five (5) years of relevant laboratory experience.
Preferred Qualifications:
- Hands-on experience with NGS workflows, including DNA/RNA sequencing, single-cell, and spatial transcriptomics assays.
- Hands-on experience with Illumina and/or Nanopore sequencing platforms.
- Strong organizational and time-management skills to maintain daily operational efficiency and meet project deadlines.
- Excellent teamwork, communication, and documentation skills.
- Experience with 10x Genomics Chromium or Visium HD platforms is highly desirable.
Work Schedule:
Full-time, Monday through Friday, 9:00 AM-5:00 PM.
Compensation:
Commensurate with education and experience.
Application Instructions:
Interested candidates should submit a cover letter, resume/CV, and contact information for three professional references.
About Us
Working at USF
With approximately 16,000 employees, the University of South Florida is one of the largest employers in the Tampa Bay area. We are dedicated to cultivating a talented, engaged and driven workforce that strives to be bold. Employees excel in USF's rich academic environment, which fosters their development and advancement. In 2025, Forbes recognized USF as one of Florida's best large employers, ranked No. 1 among the state's 12 public universities. Our first-class benefits package includes medical, dental and life insurance plans, retirement plan options, employee and dependent tuition programs, generous leave, and hundreds of employee perks and discounts.
About USF
The University of South Florida is a top-ranked research university serving approximately 50,000 students from across the globe at campuses in Tampa, St. Petersburg, Sarasota-Manatee and USF Health. USF is recognized by U.S. News & World Report as a top 50 public university and the best value in Florida. U.S. News also ranks the USF Health Morsani College of Medicine as the No. 1 medical school in Florida and in the highest tier nationwide. USF is a member of the Association of American Universities (AAU), a group that includes only the top 3% of universities in the U.S. With an all-time high of $738 million in research funding in 2024 and as a top 20 public university for producing U.S. patents, USF uses innovation to transform lives and shape a better future. The university generates an annual economic impact of more than $6 billion. USF's Division I athletics teams compete in the American Athletic Conference. Learn more at www.usf.edu.
Compliance and Federal Notices
This position may be subject to a Level 1 or Level 2 criminal background check.
Applicants have rights under Federal Employment Laws:
The University of South Florida is an equal opportunity employer that does not discriminate against any employee or applicant for employment based on any characteristic protected by law. The University maintains programs for protected veterans and individuals with disabilities in accordance with all applicable federal and state laws.
Family and Medical Leave Act (FMLA)
Applicants for USF employment are entitled to request reasonable accommodation(s) in the application process. A request is to be made at least five (5) working days prior to the time the accommodation(s) is needed. Visit the Central Human Resources ADA Accommodations webpage for more information on requesting an accommodation during the application/interview process.
Equal Employment Opportunity
The University of South Florida is an equal opportunity employer that does not discriminate against any employee or applicant for employment based on any characteristic protected by law. The University maintains affirmative action programs for protected veterans and individuals with disabilities in accordance with all applicable federal and state laws. This job description does not constitute an employment contract.