Single Cell Rna Sequencing Jobs in Philadelphia, PA

Laboratory Technician

Job Summary:  Medical Diagnostic Laboratories(MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.

MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).

MDL is looking for a Laboratory Technician. The Laboratory Technicians responsible for printing labels and attaching requisitions to the corresponding specimens.

Essential Functions: 

Accessioning:

• Print labels and attach to requisitions and the corresponding specimens
• Properly accession multiple specimen types
• Take photographs of all specimens received
• Properly identify any discrepant information
• File all paperwork in order and distribute to the appropriate QC bins
• Properly scan accessioned requisitions
• Scan 2-D barcode elution tubes used for extraction
• Properly locate and record temperatures for all necessary stations

Manual Extraction:

• Perform Phenol/Chloroform manual extractions on biopsies and ticks
• Properly separate samples into their organic and inorganic layers via phenol/chloroform
• Properly perform quantitative analysis and hydrate all ticks and biopsies

Robotic Extraction:

• Properly extract all samples types using a provided XG sheet as a template
• Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
• Properly dispose of all waste material when required; both hazardous and non-hazardous
• Attend to robotics to address any user interactions and/or robotic malfunctions
• Manually extract all necessary samples via commercial kits

RNA Extraction:

• Perform manual RNA extractions via Qiagen kit for all necessary samples
• Perform robotic RNA extractions via VX Reagents kit for all necessary samples

PCR:

• Perform manual and robotic Conventional method PCR amplification
• Properly load and start thermocycler machines
• Perform manual and robotic Real-time method PCR amplification
• Properly load and start Rotor-gene thermocycler
• Perform initial analysis on completed Real-time PCR
• Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
• Properly load and start Bio-Rad CFX thermocycler
• Perform initial analysis on completed Acoustic Real-Time PCR

 Gel Electrophoresis:

• Properly identify which completed PCRs from the thermocycler require gel electrophoresis
• Properly identify when to make and/or use 2% versus 2.5% gels
• Complete blank gel template sheets to combine short PCR sheets
• Use dye to stain all necessary samples with the appropriate volume on a test by test basis

• Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
• Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
• Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors

Pyrosequencing

• Properly perform nested PCR prior to sequencing
• Properly load and run Sequencing Analysis reactions (SQA)
• Properly load and run Single Nucleotide Polymorphism reactions (SNP)
• Properly analyze all run data

Micro-Biology/Cell Culture

• Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies
• Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs
• Properly analyze growth plate to determine antibiotic susceptibility or resistance
• For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System
• Identify and pick colonies for PCR
• Perform CAMP test and analyze for zones of hemolysis

Next-Generation Sequencing

• Properly perform a series of nested PCRs for BRCA, HPV, and Cystic Fibrosis
• Properly attach samples to appropriate bead mixtures
• Properly perform beads cleaning to remove unused dNTP’s and primers within the amplified PCR product
• Properly perform gel purification with the use of the Pippin Prep machine
• Properly load and operate multiple Life Technologies Equipment
• Properly analyze all run data

 Additional Responsibilities:

• Performs other similar or related duties as required or assigned.
• Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
• Assures customer privacy is maintained at all times.
• Participates as a team member and is accountable for own work responsibilities.
• Assures information is delivered in a manner that is supportive, timely and understandable.
• Initiative is demonstrated to proactively diagnose and resolve problems. 
• Productive work habits are consistently displayed.
• Accuracy and quality are maintained for all activities. 

Job Qualifications:  General Knowledge, Skills, and Abilities (KSA’s) required 

• 9-month Clinical Laboratory Technician training

Physical Demands: Physical, Mental and Workplace Environment Conditions

• Ability to sit, stand, walk and perform repetitive motion (manual pipette)
• Proficient visual and audio skills

Workplace Conditions: Workplace Environment Conditions

• Laboratory setting
• Use of personal protective equipment may be necessary
• May be exposed to chemicals, blood/body fluids

Education and Certifications: 

• Bachelor Degree in Biology, Chemistry, or related science field

We Offer:

•             Competitive Salary

•             Medical, Dental & Vision Insurance

•             Short and Long Term Disability

•             Life Insurance

•             Dependent Care Flex Spending Account

•             Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)

•            Identity Theft Protection

•             401(k) Plan

•             Paid Vacation & Holiday

•             Business Casual Environment

Visit us at https://www.genesisglobalgrp.com/

Genesis Biotechnology Group is a member of Genesis Global Group, an equal opportunity employer.