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AskBio Inc., a wholly owned and independently operated subsidiary of Bayer AG, is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular, central nervous system, cardiovascular, and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure, limb-girdle muscular dystrophy, multiple system atrophy, Parkinson's disease, and Pompe disease. AskBio's gene therapy platform includes Pro10, an industry-leading proprietary cell line manufacturing process, and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park, North Carolina, and European headquarters in Edinburgh, Scotland, the company has generated hundreds of proprietary capsids and promoters, several of which have entered pre-clinical and clinical testing.

Our vision: Pioneering science to create transformative molecular medicines.

Our mission: Lead innovative science and drive clinical outcomes to transform people's lives.

Our principles:

• Advance innovative science by pushing boundaries.

• Bring transformative therapeutics to patients in need.

• Provide an environment for employees to reach their fullest potential.

Our values:

• Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.

• Cultivate Collaboration. Strive to be the best teammate, actively listen, openly communicate, and embrace diverse points of view.

• Embrace Responsibility. We are humbled by the enormity of our mission.We hold a relentless commitment to advance science and clinical outcomes for our patients, families, and caregivers.

• Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.

• Act with Uncompromising Integrity. Be honest, transparent, and committed to doing what's right in every situation. Make clear commitments and follow through.

Position Summary

AskBio is seeking a motivated, detail-oriented, and collaborative Research Associate II to support AAV delivery research within the Delivery organization in R&D. This role provides hands-on experimental support across molecular biology and cell-based workflows that advance AskBio's preclinical gene therapy pipeline. The Research Associate II will work closely with scientists within the Delivery team to execute established experimental protocols with increasing independence, generate high-quality and reproducible data, and support day-to-day laboratory operations.

This position is based in Morrisville, North Carolina.

Job Responsibilities

• Perform plasmid purification, genomic DNA extraction, RNA extraction, and PCR-based assays (PCR, qPCR, ddPCR)

• Conduct routine mammalian cell culture activities, including maintenance of adherent cell lines and execution of cell-based assays following established SOPs

• Perform in vitro assays including transfection and AAV transduction

• Support in vitro and in vivo studies through sample preparation, tissue processing, and downstream molecular analyses

• Accurately document experimental procedures and results in an electronic laboratory notebook in accordance with data integrity standards

• Analyze data, summarize results, and communicate findings through internal reports or presentations

• Maintain laboratory organization, including reagent preparation, inventory management, and inspection readiness

• Contribute to continuous improvement of laboratory workflows by following SOPs and escalating issues appropriately

Minimum Requirements

• Bachelor's degree in Molecular Biology, Biochemistry, Biotechnology, or a related scientific field with 2+ years of relevant laboratory experience; OR a Master's degree in Molecular Biology, Biochemistry, Biotechnology, or a related scientific field

• Hands-on experience with molecular biology techniques, including DNA/RNA isolation, SDS-PAGE, qPCR, ddPCR and Western blot

• Prior experience with mammalian cell culture and aseptic technique

• Strong technical laboratory skills with the ability to independently execute protocols and experiments

• Ability to follow established protocols with minimal supervision and deliver reliable, reproducible results

• Strong organizational skills, attention to detail, and commitment to accurate documentation

• Ability to manage multiple tasks simultaneously and work effectively within a collaborative, crossfunctional team environment

• Proficiency with basic data analysis and common laboratory software tools (e.g., Excel, GraphPad)

Preferred Education, Experience and Skills

• Experience supporting AAV research or delivery programs, including familiarity with viral vectors or gene therapy concepts

• Exposure to AAV production or purification workflows at a research scale

• Experience preparing samples for downstream analyses such as NGS or other advanced molecular assays

• Comfort working across diverse experimental workflows and learning new techniques as project needs evolve

AskBio Inc. (AskBio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race, color, religion, gender, sexual orientation, gender identity, national origin, age, disability, veteran status or any other protected status prohibited under Federal, State or local laws. All employment decisions are based on valid jobrelated requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website, you may request a reasonable accommodation to express interest in a specific opening by calling us at (919) 561-6210 or sending us an email at careers@askbio.com.

Agencies: Please do not contact any employee at AskBio about this requisition. Any resume submitted by a recruitment agency to any employee at AskBio, through any medium, will be deemed the sole property of AskBio unless the agency was engaged by AskBio Talent Acquisition team to recruit for that position. All agencies must have a prior executed service agreement with AskBio prior to any search engagement. If a candidate who was submitted outside of the AskBio agency process is hired by AskBio, no fee or payment of any kind will be paid to the agency.