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Enzyme Assay Development Jobs (NOW HIRING)

Bench Scientist

Groton, CT · On-site

$35 - $44/hr

Experience in assay development and mechanisms of enzyme inhibition is preferred. * Experience with automation of assays and LC-MS is also desired SKILLS: * Experience conducting in vitro biology ...

Biologist

Boston, MA · On-site

$83 - $88/hr

In this role, the candidate will support assay development and perform established assays to ... or GPCRs, enzymes, transporters, etc.) and multiple modalities (small molecules, peptides ...

Senior Scientist, Assay Research

Austin, TX · On-site

$97K - $124K/yr

You bring deep expertise in microbial metabolism, enzymology, or cell-based signaling and know how ... Technology Development * Benefits: Health insurance, 401k matching, PTO, and the chance to make a ...

Senior Scientist, Analytical R&D

Rahway, NJ · On-site

$100K - $127K/yr

Experience with large molecule characterization (oligonucleotides, enzymes, peptides, etc ... Adaptability, Analytical Method Development, Assay Development, Bioanalytical Analysis, Biochemical ...

Senior Scientist, Analytical R&D

Rahway, NJ · On-site

$100K - $127K/yr

Experience with large molecule characterization (oligonucleotides, enzymes, peptides, etc ... Adaptability, Analytical Method Development, Assay Development, Bioanalytical Analysis, Biochemical ...

Scientist III - Potency Assay - FSP

Three Rivers, MI · On-site

$32.50 - $40.75/hr

Each of us, no matter what we do at Parexel, contributes to the development of a therapy that ... Experience with plate-based potency assays (cell-based assays, enzyme-linked immunosorbent assay ...

Research Professional II

Tucson, AZ · On-site

$15.50 - $21.25/hr

Conduct experiments in support of research projects, contributing to hypothesis development and ... Perform and refine molecular, biochemical, and functional assays - including enzyme assays, protein ...

Principal Scientist

San Diego, CA · On-site

$149K - $181K/yr

... enzymology, or a related scientific discipline. * A consistent track record of impact on drug ... Proficiency in biochemical assay development including proximity-based assays (such as TR-FRET and ...

Completion of a Master's thesis focused on proteins (e.g., enzyme kinetics, protein-protein ... The successful candidate will have 0-3+ years of experience working in assay development of ...

ML-guided iterative enzyme evolution Novel recombinase testing and optimization Molecular biology assay development & execution for high-throughput DNA assembly Lastly, an important aspect woven into ...

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Enzyme Assay Development information

See salary details

$29K

$53.4K

$81K

How much do enzyme assay development jobs pay per year?

As of Jun 20, 2026, the average yearly pay for enzyme assay development in the United States is $53,418.00, according to ZipRecruiter salary data. Most workers in this role earn between $42,500.00 and $60,000.00 per year, depending on experience, location, and employer.

What are some common challenges faced when developing enzyme assays, and how can new team members effectively address them?

A common challenge in enzyme assay development is ensuring assay sensitivity and specificity while maintaining reproducibility across experiments. New team members can address these challenges by rigorously optimizing assay conditions, carefully selecting substrates and controls, and consistently documenting protocols. Collaborating closely with colleagues in analytical chemistry and biostatistics can also help troubleshoot technical issues and interpret complex data, leading to more robust and reliable assays.

What jobs use PCR?

Enzyme assay development jobs often utilize PCR (Polymerase Chain Reaction) to amplify DNA for analysis, cloning, or quantification. Skills in molecular biology techniques, including PCR, are essential for roles involving genetic research, diagnostics, or assay optimization in laboratory settings.

What are the key skills and qualifications needed to thrive in Enzyme Assay Development, and why are they important?

To thrive in Enzyme Assay Development, you need a solid background in biochemistry or molecular biology, with experience in enzymology and assay design often supported by an advanced degree. Familiarity with laboratory techniques such as spectrophotometry, fluorescence assays, and data analysis software, as well as knowledge of high-throughput screening systems, is crucial. Attention to detail, problem-solving abilities, and clear communication help in troubleshooting experiments and collaborating with multidisciplinary teams. These competencies ensure reliable, reproducible assay results that are essential for drug discovery, diagnostics, and research success.

How to develop an enzyme assay?

Enzyme assay development involves selecting an appropriate substrate, optimizing reaction conditions such as pH and temperature, and establishing a reliable detection method like spectrophotometry or fluorometry. It requires understanding enzyme kinetics and often involves validating the assay's specificity, sensitivity, and reproducibility to ensure accurate measurement of enzyme activity.

What does an enzyme assay do?

An enzyme assay is a laboratory test used by enzyme assay development professionals to measure the activity of enzymes in a sample. It helps determine enzyme function, efficiency, and stability, often using spectrophotometry or other analytical tools to analyze reaction rates and substrate conversion. This information is essential for developing, optimizing, and validating enzyme-based products or research applications.

What is the difference between Enzyme Assay Development vs Biochemist?

AspectEnzyme Assay DevelopmentBiochemist
Required CredentialsBachelor's or Master's in Biochemistry, Molecular Biology, or related fieldsBachelor's or higher in Biochemistry, Chemistry, or related fields
Work EnvironmentLaboratories focused on assay design, validation, and optimizationResearch labs, pharmaceutical companies, or academic institutions
Industry UsagePrimarily in biotech, pharma, and research organizationsBroadly in research, development, and academia
Common Search/ComparisonYesYes

Enzyme Assay Development specialists focus on creating and validating assays to measure enzyme activity, often working on specific test methods. Biochemists have a broader scope, studying biological molecules and processes, which may include enzyme research but also extends to other areas. Both roles require similar educational backgrounds and often work in similar environments, but their focus and responsibilities differ.

What is enzyme assay development?

Enzyme assay development is the process of designing and optimizing laboratory tests to measure the activity of specific enzymes. These assays are essential for studying enzyme kinetics, screening for inhibitors or activators, and monitoring biochemical reactions in research and drug discovery. Developing an enzyme assay involves selecting appropriate substrates, reaction conditions, and detection methods to ensure accurate and reproducible results. This process often requires troubleshooting and validation to ensure the assay is sensitive, specific, and suitable for its intended application.

What does an assay development scientist do?

An assay development scientist designs, optimizes, and validates laboratory tests to measure enzyme activity or other biological molecules. They work with techniques such as spectrophotometry, chromatography, and molecular biology tools, often following Good Laboratory Practice (GLP) standards, to ensure assays are accurate, reliable, and suitable for research or diagnostic purposes.
Infographic showing various Enzyme Assay Development job openings in the United States as of June 2026, with employment types broken down into 97% Full Time, 1% Temporary, and 2% Contract. Highlights an 60% Physical, 1% Hybrid, and 39% Remote job distribution, with an average salary of $53,418 per year, or $25.7 per hour.
Development Scientist I/II, Automation

Development Scientist I/II, Automation

New England Biolabs

Ipswich, MA • On-site

$85K - $100K/yr

Full-time

Retirement

Posted 25 days ago


Job description

About NEB

New England Biolabsis a different kind ofbiotechnology company - we areacommunity of scientists, innovators and collaborators driven by a shared mission to advance scienceforthe benefit ofsociety. Founded in 1974 with a deep commitment to research excellence and environmental stewardship, NEB has become a global leader in the discovery and production of enzymes for molecular biology applications. Our reagents are trusted by researchers worldwide for their quality and reliability and have played a critical role in breakthroughs across genomics, proteomics, diagnostics and academic research. With acorporateculture rooted in passion, humility and authenticity, we offer a dynamic and inclusive workplace where creativity thrives, voices are heard, and personal growth is celebrated. Whether you are interested in pursuing a research career or supporting our missionthrough other roles,joining NEB means contributing to meaningful scientific progress in a collaborative, campus-like environment that values both innovation and integrity.

The role

We are inviting applications for the position of Automation Development Scientist I/II. The successful candidate will work as a member of the Applications and Product Development Department. This individual will be responsible for designing, developing, optimizing, and maintaining laboratory automation workflows in support of product development activities at NEB. This position offers opportunities to strategize, optimize processes, learn from colleagues and align with other groups and departments across the company to deliver impactful solutions.


How you'll contribute:

  • Design, build, and validate end-to-end laboratory automation protocols for highthroughput NGS sequencing/other technologies using liquid handlers, and other integrated instrumentation

  • Optimize existing workflows to enhance reliability, reproducibility, precision, and throughput

  • Troubleshoot automation performance issues across hardware, software, and functional assays

  • Implement and maintain high throughput automation platforms

  • Evaluate new automation technologies and lead pilots or onboarding of new equipment.

  • Partner with SMEs to translate manual assays into automated methods.

  • Develop documentation, SOPs, and training materials for automated workflows.

  • Provide hands-on training and support to lab teams on automated protocols, platforms and best practices

  • Assist customers with technical inquiries regarding NEB products

  • Work closely across the Applications and Development teams and other departments to integrate automation solutions that accelerate discovery

Required Qualifications and Experience:

  • PhD in biochemistry, molecular biology, or related field with minimum 2 years of experience (or master's with 5 years of experience)

  • Experience in protocol design, troubleshooting and implementation on various liquid handling platforms (such as Agilent Bravo, Hamilton, Revvity, Beckman, SPT Labtech, etc.)

  • Working knowledge of LIMS/ELN integration, data pipelines, and experiment tracking systems

  • Have "good hands" with proven bench skills

  • Strong ability to multi-task, self-manage and perform effectively in a fast-paced team

  • Excellent communication and interpersonal skills

  • Be goal and deadline oriented with exceptional attention to detail

  • Have a strong work ethic, emphasizing integrity, efficiency and quality of work

What will make you stand out:

  • Experience in molecular biology techniques, high-throughput screening (HTS) or assay development preferred

  • Knowledge of Next Generation Sequencing workflows preferred

Estimated base salary hiring range for a Development Scientist I/II, Automation is $85,000 - $100,000

Base salary is onecomponent of our generous total rewards compensation package. Employees are eligible to receive:

  • Annual discretionary profit-sharing bonus
  • Participation in our Employee Stock Ownership Plan "ESOP" (eligible after two full years of service)
  • Exceptional benefits package at a nominal cost to the employee including a 10% 401k match

Benefits at NEB

Base salary decisions depend on several factors, including, but not limited to, individual qualifications, job location, internal equity, and market data alignment.

Equal Opportunity Employer, including disabled/veterans

New England Biolabs is committed to fostering a diverse and inclusive community. As an Equal Employment Opportunity employer, New England Biolabs considers applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, or status as a disabled veteran or other protected veteran, in all phases of the employment process and in compliance with applicable federal, state, and local laws and regulations. More in-depth details of EEO are availablehere.

Information regarding New England Biolabs' commitment to Diversity, Equity, and Inclusion can be foundhere.

New England Biolabs is a participating employer in the Employment Verification(E-Verify)program.

If you need an accommodation for any part of the employment process because of a medical condition or disability, please send an email tohr@neb.comor call 978-927-5054 to let us know the nature of your request.