Entry Level Tissue Culture Jobs (NOW HIRING)

University of Colorado Anschutz Medical Campus
Department: Pharmacology
Job Title: Research Services Professional (Open Rank: Entry - Principal)
Position # 00850276- Requisition #40415
Job Summary:
As a Research Services Professional in the Brambati Lab, you will gain valuable new skills at the bench while leveraging your strong organizational skills to manage a new and growing research group within the Department of Pharmacology at the University of Colorado School of Medicine's Anschutz Medical Campus. A central responsibility of this position is serving as the tissue culture lead for the lab: maintaining cell lines, organizing supplies and reagents, upholding standard procedures, and managing iPSC culture and neuronal differentiation. No prior experience with iPSCs is required; full training will be provided. Any prior tissue culture experience is a plus.
This position is open to all qualified and motivated individuals and may be of particular interest to those preparing to apply to medical or graduate school. The lab environment provides exposure to a broad array of concepts and techniques related to molecular biology research on DSB repair using cancer cells and stem cells as a model system. Our goal is to put your existing skills to use while exposing you to new idea and techniques that benefit your career goals and the lab's overall productivity.
Key Responsibilities:
Entry Level

• Collaborate with and support Principal Investigators (PI) and other stakeholders with laboratory, research, and administrative functions.
• Learn and utilize a variety of laboratory techniques required for research projects. Depending on existing expertise and personal interests, these techniques may include:
  
  • Transformation, bacterial culture, and mini/midi-preps for plasmid DNA purification.
  • PCR, DNA digestion, and gel electrophoresis.
  • Western blotting.
  • CRISPR-Cas9-based assays and screening.
  • Tissue culture management: serve as the lab's tissue culture lead under training and supervision. Organize tissue culture supplies and reagents and maintain all cell lines, with a particular focus on stem cell preparation, induced pluripotent stem cells (iPSCs), and their differentiation into neurons. No prior iPSC experience is required; training will be provided. Any prior tissue culture experience is considered a plus.
  • Immunostaining for protein localization studies.
  • Confocal microscopy for high-resolution imaging.
  • Generation of CRISPR-Cas9 knockout (KO) human cell lines.
• You do not need to be an expert in all techniques listed, although it would be helpful if you are familiar with some of them.
• Prepare lab for daily operations by ensuring safety and cleanliness.
• Manage and maintain laboratory reagents and inventory.
  
  • Manage procurement via departmental software platform.
  • Maintain inventories of critical lab reagents and equipment.
  • Handle delivering, receiving, and retrieving frozen samples from freezers and physical inventory of frozen samples in -20 and -80C freezers.
  • Coordinate equipment maintenance as necessary.
• Dispose of laboratory materials and samples using well-established guidelines and instructions.
• Review and implement lab support policies, practices, and programs to meet organizational, operations, and management needs.
• Ensure compliance with applicable laws, rules, and regulations; receive guidance and direction or escalate when required.

Intermediate Level (in addition to all Entry-level duties)

• Independently and creatively identify laboratory solutions.
• Independently review, develop, and implement new or modified laboratory techniques required for research projects.
• Assist Team Leads, Supervisors, and/or management with creation and implementation of processes and procedures and quality improvement initiatives.
• Assist and train junior team members.

Senior Level (in addition to all Intermediate-level duties)

• Assist Team Leads, Supervisors, and/or management with creation and implementation of processes and procedures and quality improvement initiatives.
• Assist and train junior team members.
• Assist with developing or develop protocol-specific systems and documents including process flows, training manuals, and Standard Operating Procedures (SOPs). Maintain experimental documentation and prepare protocols, equipment, and supplies.
• Provide laboratory science expertise and leadership in study design, experimental oversight, data management, data analysis, and manuscript preparation to assist all levels of investigators and researchers.

Principal Level (in addition to all Senior-level duties)

• Act as a Subject Matter Expert and authority in the area(s) of DNA repair and/or stem cell biology.
• Assist the PI with design, writing, and preparation of funding applications.
• Oversee and provide informal leadership to team members, including managing day-to-day priorities, evaluating and redistributing workloads on a temporary basis, and mentoring staff.
• Provide input and feedback to leadership on team members' overall performance, performance evaluations, opportunities for development, and process improvement initiatives.
• Identify training and development opportunities for new and existing team members.

Additional Responsibilities, All Levels

• Presenting data or updating projects at weekly lab meetings and/or one-on-one with the PI.
• Support and assist team members and researchers from collaborative groups on research projects, experimental designs, and laboratory-based methodologies.
• Assist in all other projects as needed.

This description is a summary only and is describing the general level of work being performed; it is not intended to be all-inclusive. The duties of this position may change from time to time and/or based on the laboratory need. We reserve the right to add or delete duties and responsibilities at the discretion of the supervisor and/or hiring authority.
Work Location:
Onsite - this role is expected to work full-time onsite and is located in Aurora, Colorado.
Why Join Us:
The Brambati lab is focused on uncovering the molecular mechanisms that regulate DNA repair and genome instability, with a special emphasis on non-canonical DNA double-strand break (DSB) repair pathways. Our research integrates cutting-edge approaches in genetics, cell biology, and molecular biology to address key biological questions that are fundamental to understanding genome maintenance.
By joining our dynamic and rapidly growing lab, you will gain hands-on experience with a wide range of advanced experimental techniques. As a new lab, we offer a unique opportunity to contribute to the building and shaping of our research environment, a valuable experience for anyone considering a future career as an independent scientist.
Why Join Us?

• Innovative Research: Work on groundbreaking projects that aim to solve important problems in genome stability and repair mechanisms.
• Mentorship and Growth: Dr. Brambati is deeply committed to mentoring, with a personalized approach to support the individual goals of each lab member. Whether you aim to pursue a career in academia, industry, or another path, we provide the resources and guidance to help you succeed.
• Collaborative Environment: Our lab fosters an inclusive and supportive atmosphere where young scientists can grow, share ideas, and gain independence.
• Cutting-edge Techniques: You'll have the opportunity to learn and apply advanced methods spanning molecular biology, cell biology, imaging, and more.

If you're passionate about understanding genome stability and aspire to make significant contributions to the field, the Brambati Lab is the perfect place for you!
Why work for the University?
We have AMAZING benefits and offerexceptional amounts of holiday, vacation and sick leave! The University of Colorado offers an excellent benefits package including:

• Medical: Multiple plan options
• Dental: Multiple plan options
• Additional Insurance: Disability, Life, Vision
• Retirement 401(a) Plan: Employer contributes 10% of your gross pay
• Paid Time Off: Accruals over the year
• Vacation Days: 22/year (maximum accrual 352 hours)
• Sick Days: 15/year (unlimited maximum accrual)
• Holiday Days: 10/year
• Tuition Benefit: Employees have access to this benefit on all CU campuses
• ECO Pass: Reduced rate RTD Bus and light rail service

There are many additional perks & programs with the CU Advantage.
Qualifications:
Minimum Qualifications
Entry Level:

• Bachelor's degree in biology, neuroscience, biochemistry, biomedical sciences, or related field.
• A combination of education and related technical/paraprofessional experience may be substituted for the bachelor's degree on a year for year basis.

Intermediate Level:

• Bachelor's degree in biology, neuroscience, biochemistry, biomedical sciences, or related field.
• A combination of education and related technical/paraprofessional experience may be substituted for the bachelor's degree on a year for year basis.
• One (1) year of professional level research experience.

Senior Level:

• Bachelor's degree in biology, neuroscience, biochemistry, biomedical sciences, or related field.
• A combination of education and related technical/paraprofessional experience may be substituted for the bachelor's degree on a year for year basis.
• Two (2) years of professional level research experience.

Principal Level:

• Bachelor's degree in biology, neuroscience, biochemistry, biomedical sciences, or related field.
• A combination of education and related technical/paraprofessional experience may be substituted for the bachelor's degree on a year for year basis.
• Three (3) years professional level research experience.

Applicants must meet minimum qualifications at the time of hire.
Preferred Qualifications

• Master's degree in biology, neuroscience, biochemistry, biomedical sciences, or related field.
• Prior experience or background within an experimental or wet lab.
• Prior mammalian tissue culture experience (experience with iPSCs or stem cells is a plus but not required).

Principal Level:

• Doctoral degree in biology, neuroscience, biochemistry, biomedical sciences, or related field.

Conditions of Employment

• Applicants must be legally authorized to work in the United States without requiring sponsorship. We are unable to provide work visa sponsorship or employment authorization for this position now or in the future.

Knowledge, Skills, and Abilities

• Ability to communicate effectively, both in writing and orally.
• Ability to organize diverse administrative and research tasks to reliably meet deadlines.
• Ability to establish and maintain effective working relationships with employees at all levels throughout the institution.
• Ability to interpret and master complex research protocol information.
• Knowledge of laboratory techniques including safety procedures and techniques.

How to Apply:
For full consideration, please submit the following document(s):
A letter of interest describing relevant job experiences as they relate to listed job qualifications and interest in the position (max 1 page).
Curriculum vitae / Resume (max 2 page).
Two or three professional references, including name, affiliation, address, phone number (mobile number if appropriate), and email address.
Questions should be directed to: Jennifer Orsund, jennifer.orsund@cuanschutz.edu
Screening of Applications Begins:
Screening of applications begins July 1st and continues until the position is filled.
Anticipated Pay Range:
The starting salary range (or hiring range) for this position has been established as:

• Entry Level: $47,811 to $54,067
• Intermediate Level: $52,030 to $69,720
• Senior Level: $56,249 to $75,373
• Principal Level: $64,686 to $86,679

The above salary range (or hiring range) represents the University's good faith and reasonable estimate of the range of possible compensation at the time of posting. This position may be eligible for overtime compensation, depending on the level.
Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave, health plans and retirement contributions that add to your bottom line.
Total Compensation Calculator: http://www.cu.edu/node/153125
Equal Employment Opportunity Statement:
CU is an Equal Opportunity Employer and complies with all applicable federal, state, and local laws governing nondiscrimination in employment. We are committed to creating a workplace where all individuals are treated with respect and dignity, and we encourage individuals from all backgrounds to apply, including protected veterans and individuals with disabilities.
ADA Statement:
The University will provide reasonable accommodations to applicants with disabilities throughout the employment application process. To request an accommodation pursuant to the Americans with Disabilities