Broadly neutralizing antibodies can protect against HIV-1 infection in animal models. The Saunders laboratory investigates the antibody response to broadly neutralizing epitopes on HIV-1 Envelope protein in the context of Envelope vaccination. Many of the broadly neutralizing epitopes involve N-linked glycosylation, which is a central focus of the Saunders laboratory’s research. The candidate will
- Perform the isolation and characterization of HIV-1 antibodies from vaccinated human and animal models.
- Work independently to perform immunoassays, fluorescence-activated single B cell sorting, and antibody cloning. Characterize the binding and neutralization phenotypes of monoclonal antibodies identified by cell sorting and next generation sequencing.
- Investigate the ontogeny of vaccine-induced neutralizing antibodies. Under the advisement of Dr. Saunders, the candidate will be responsible for designing and executing experiments, interpreting data, and supervising junior scientists.
- Develop and optimize new assays for the laboratory
- Perform the following experimental activities:
- Plasmid sequence QC for on-going protein production needs.
- Large scale antibody and HIV-1 envelope production and purification.
- Large scale cell culture.
- QC recombinant antibodies and HIV-1 envelopes using biochemical and immunological techniques, such as FPLC, SDS-PAGE, Western blots and ELISA
- Fluorescence-activated cell sorting of single B cells from vaccinated humans and animal models.
- PCR amplification of antibody genes.
- Expression of recombinant HIV-1 antibodies by transient transfection.
- HIV-1 envelope and antibody sequence analysis.
- Immunoassays to include (luminex, ELISA, and Western Blots).
- Molecular biology assays such as PCR, site-directed mutagenesis, DNA preparation.
- Train laboratory staff and DHVI trainees.
- Compliantly order materials and reagents for ongoing projects in the lab.
- Maintain a detailed and accurate laboratory notebook.
- Perform preventative maintenance on laboratory equipment to ensure proper function.
- Manage accounts with external vendors and internal core facilities.
- Perform other related duties as necessary and requested.
Required Qualifications at this Level
- Education/Training: Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
- Experience: Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Duke Human Vaccine InstituteThe Duke Human Vaccine Institute (DHVI) continues to lead with cutting edge vaccine research against infectious diseases that impact global health. The investigators at the DHVI conduct basic and translational research to develop novel vaccines, therapeutics and diagnostics for diseases such as HIV-1, tuberculosis, influenza, malaria, ebola, cytomegalovirus and now the zika flavivirus. Several DHVI investigator led basic science discoveries are currently being produced in Good Manufacturing Practice (CGMP) facilities for early phase vaccine trials.